C785 BIOCHEMISTRY FINAL EXAM STUDY GUIDE

C785 BIOCHEMISTRY FINAL EXAM STUDY GUIDE What is the basic structure of an amino acid? List the 4 groups and describe what they Tyr Val Tyr Ile Gln Ile Ile Asp Val Leu His Lys 3. Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? * 0/1 Feedback The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T --- U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' --- 3') Tyr Tyr Arg Ile Met Ala Ser Val Ile Ser His Gln 4. Which of the following are the correct components for a PCR reaction? * 1/1 dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA ATP, Primer, mRNA polymerase; template mRNA Correct answer Ser Val Ile Feedback The correct answer is C because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile Acetyl CoA, RNA primer, DNA Ligase, Template phosphate 5. If you had a mother who is homozygous for a recessive disease, and a father who is a heterozygous carrier, what percentage of children will have the disease? * 1/1 0% 25% 50% 75% 100%