CMMB Tutorial Quiz 1 well answered graded A+ 2024/2025

CMMB Tutorial Quiz 1WISH - correct answer Whole-mount Insitu Hybridization - involves staining whole embryos with complementary RNA probe with label (DIG). Probe is detecting using anti-DIG antibody. *Causes embryos to shrink B-Galactosidase/LacZ reporter assay - correct answer Dna containing enhancer, promoter and reporter injected into embryo creating a transgenic animal. LacZ reporter encodes B-galactosidase enzyme that reacts with substrate addition. Location of staining = location of enhancer activated LacZ expression Cre/LoxP system - correct answer Conditional removal of DNA sequences from genome. Cre bacterial enzyme that can excise floxed genes (flanked by LoxP sites) and cell can no longer express that gene. Animals are considered "conditional mutants" Cre - correct answer Bacterial enzyme that can excise floxed genes LoxP - correct answer Short, specific DNA sequences that are situated on either side of a specific DNA sequence containing gene that is targeted for removal B-galactosidase - correct answer Enzyme encoded by LacZ reporter gene that reacts with a specific substrate and stains portion of the embryo LacZ - correct answer Reporter gene that encodes B-galactosidase enzyme Hox genes - correct answer Exhibit collinearity in which chromosomal sequences mirrors placement on the body axis of the embryo (Hox1-13) Hoxb1 - correct answer Hindbrain expression Hoxa11 - correct answer Middle limb segments and tail vertebrae (zeugopod & tail expression)