Cgmbs - Study guides, Class notes & Summaries

What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction enzymes 2. Fragments are separated by gel electrophoresis 3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), complementary to region of interest What is RFLP? - ️️- Restriction Fragment Length Polymorphism - The number and location of restriction sites for a given restriction enzymes are not the same in all individuals What is the mapping difference between linear DNA f...

Which of the following polymorphisms are tandem repeats of 1-8 bp? a. Single nucleotide polymorphisms b. Variable number tandem repeats c. Short tandem repeats d. Restriction fragment length polymorphisms → Short tandem repeats Which of the following is used as a marker of gender? a. D8S1179 b. Amelogenin c. TPOX d. Penta D → Amelogenin What is an expression for how many times more likely the child's allele is inherited from the alleged father than by random occurren...

BCR;ABL1 fusion protein → t(9;22)(q34;q11) Acute Promyelocytic Leukemia (APL) → t(15;17) a fusion of PML and RARA Burkitt's Lymphoma → t(8;14) the breakpoints on chr.8 are spread of 190 kbp region 5' to and within the CMY C gene Follicular Cell Lymphoma (FCL) → t(14;18) translocation disregulate BCL2, which inhibits apoptosis in B lymphocytes, which leads to survival of genetically damaged cells Mantle Cell (MCL) → t(11;14) translocation increases expression of CCND1 ...

ASCP CGMBS Exam Study Questions and Complete Solutions Graded A+