Cgmbs - Study guides, Class notes & Summaries

CGMBS EXAM 1 (STUDY) QUESTIONS WITH SOLVED ANSWERS GRADED A+

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CGMBS EXAM 5 (2024) QUESTIONS WITH PASSED SOLUTIONS!!

DNA polymerase delta - Answer-eukaryote: completes replication on the *lagging* strand; binds more tightly to DNA sliding clamp nucleotide and base excision repair Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping them from re-pairing topoisomerase 1 - Answer-Making single-stranded DNA breaks to relieve supercoiling at origin topoisomerase 2 - Answer-An enzyme that breaks both strands (a DNA double helix), rotates the ends, and seals the break. *USES ATP*...

Sequencing of the DNA region encoding 16S rRNA is performed to determine - ️️the evolutionary and genetic relatedness of microorganisms and has driven changes in microorganism nomenclature Homologous extrinsic control - ️️target-derived control with a non-target-derived sequence insert; control is added to every sample after nucleic acid extraction and before amplification Smallpox must be handled only in approved laboratories - ️️level 4 containment When processing whole bloo...

Warfarin (Coumadin) - ️️CYP2C9 and VKORC1 - Vitamin K antagonist; metabolized primarily via oxidation in the liver by CYP2C9 and exerts its anticoagulant effect by inhibiting the protein vitamin epoxide reductase complex, subunit 1 (VKORC1) Chronic Myelogenous Leukemia (CML) - ️️t(9;22) aka Philadelphia Chromosome, translocation occurs within two genes: the breakpoint cluster region (BCR) on chr.22 and the cellular counter part of the Abelson Leukemia virus tyrosine kinase (c-abl) o...

topoisomerase 1 → Making single-stranded DNA breaks to relieve supercoiling at origin topoisomerase 2 → An enzyme that breaks both strands (a DNA double helix), rotates the ends, and seals the break. → *USES ATP* DNA gyrase → type of topoisomerase 2 DNA helicase → An enzyme that unwinds the DNA double helix at origin Primase → RNA polymerase: An enzyme that joins RNA nucleotides to make the primer using the parental DNA strand as a template. Single strand binding prote...

DNA was isolated from a patient and cut with HaeIII. The resulting fragments were separated by gel electrophoresis and transferred to a membrane. The fragments were allowed to hybridize to a probe, and the resulting band pattern was compared with that seen in another sample of DNA. Which of the following methods is described? a. RFLP by southern blot b. VNTR by PCR c. SNP by sequencing d. STR by PCR - ️️RFLP by southern blot Which of the following is used as a marker of gender? a. D...

Salmonella typhi specimen collection → initially present in peripheral bloo but not in urine or stool util at least 2 weeks after infection Smallpox must be handled only in approved laboratories → level 4 containment When processing whole blood, why is it important to remove homoglobin → can inhibit DNA polymerase and prevent amplification of nucleic acid resulting in false-negative PCR result Inhibitors or DNA pol. in urine → -nitrate → -crystals → -hemoglobin → -bet...