Totect Study guides, Class notes & Summaries

Periodic Acid Schiff stain demonstrates CORRECT ANSWER-Polysaccharides, neutral mucosubstances, and basement membranes Periodic acid and Chromic acid oxidise carbohydrates to CORRECT ANSWER-aldehydes What acid causes loss of quinoid structure in basic fuchsin? CORRECT ANSWER-Sulfurous acid The control of PAS is CORRECT ANSWER-Kidney The control for PAS-D is CORRECT ANSWER-Liver - one section "with" and one section "without" Alcian blue pH 2.5 demonstrates CORRECT ANSWER-Acid m...

BOC Histotechnology- Processing During microtome, it is noted that many of the tissues are very hard and shrunken. Of the following, the most likely explanation for this problem is that the: - correct answers INFILTRATING PARAFFIN IS TOO HOT. When tissue is processed in paraffin wax that is more than 2 degrees Celsius to 4 degrees Celsius above the melting point, the tissue becomes hard and shrunken. Decalcification of small specimens can be achieved by fixation in: - correct answers ZENK...

affinity or avidity:a dye is attracted to a tissue CORRECT ANSWER-affinity affinity or avidity: how strongly a dye is attracted to a tissue CORRECT ANSWER-avidity what happens when amphoteric tissue (or dye) is placed in an acidic solvent? CORRECT ANSWER-becomes basic what happens when amphoteric tissue (or dye) is placed in a basic solvent? CORRECT ANSWER-becomes acidic what defines "how fast a dye stains" CORRECT ANSWER-vigour what type of sectionned tissue stains the fastest?...

Absorption - correct answers When tissue is penetrated by, or absorbs, a dye solution and becomes colored without any other change or chemical reaction occurring. An example is the absorption of oil red O or Sudan black B by fat Achromatic - correct answers Without color, or not easily stained. In microscopy, achromatic lenses are corrected for two colors, red and blue. This helps produce images free of chromatic aberrations Acid mucosubstances - correct answers a term used to include bot...

formalin CORRECT ANSWER-Which of the following pigments is birefringent? A. formalin B. bile C. lipofuscin D. melanin 8 - 10 micrometer CORRECT ANSWER-What micrometer thickness is preferred for the technique shown? skeletal CORRECT ANSWER-Muscle that histologically contains cytoplasmic cross-striations and has multiple nuclei located at the edge of the fibers is classified as: Von kossa CORRECT ANSWER-The technique shown below is most likely: Schmorl Stain CORRECT ANSWER-The tech...

An example of an additive fixative is one that contains: CORRECT ANSWER-picric acid Microscopic examination of an H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following: CORRECT ANSWER-incomplete fixation Microscopic evalution of H&E stained sections from a surgically removed small bowl specimen shows an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from: CORRECT...

Doxorubicin Cardiotoxic TISSUE VESICANT Baseline MUGA and total dose (CONCERN at 550 mg/m2) URINE=RED IF EXTRAVASATION OCCURS= cool/ice + Totect Idamycin Cardiotoxic (cardiomyopathy) Bleomycin AVOID oxygen supplementation Methotrexate Leukovorin decreases cytotoxicity Pemetrexed Vitamin B12 & folic acid supplements to decrease toxicity Fludarabine Immunosuppression Thioguanine Bone Marrow Suppression Stomatitis/Mucositis 5-FU *Stomati...

What are the steps tissue must go through before it is viewed under a microscope? CORRECT ANSWER-1. Fixation 2. Processing 3. Embedding 4. Microtomy 5. Staining 6. Coverslipping Define fixation. CORRECT ANSWER-The preservation of cells and tissue in as life-like a way as possible by stabilizing the protein so that it is resistant to further changes. What are the functions of fixation? CORRECT ANSWER-1. To prevent putrefaction and autolysis 2. To help maintain proper relationships b/w...

Microscope - correct answers Used to for the magnification of the tissues and cells. Microtome - correct answers Used for the cutting of the wax and tissues. Block holder (chuck) - correct answers Holds the tissue in position. Knife and Knife carrier - correct answers For actual cutting of tissue sections. Pawl, Ratcher Feed Wheel & Adjustment screw - correct answers Lines up the tissue block in proper position with knife, adjusting thickness of tissues for successive sections. So...

For adequate fixation, the fixative volume should be at least ______ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 CORRECT ANSWER-D. 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid. A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope CORRECT ANSWER-B. Polarizing microscope For most routine H & E staining, the sections should be 1 cel...