Totect Study guides, Class notes & Summaries

What are the steps of processing? CORRECT ANSWER-Fixation, (decalcification), dehydration, clearing, infiltration, embedding The step of processing removes water for the next subsequent step to occur (xylene and paraffin) CORRECT ANSWER-dehydration What accounts for the majority of tissue processing problems? **** CORRECT ANSWER-incomplete dehydration How does one solve incomplete dehydration? CORRECT ANSWER-Reprocess in water and then absolute alcohol. Or melt paraffin block, pat tissu...

What method is used to stabilize proteins making it resistant to further damage? CORRECT ANSWER-Fixation Name two methods of fixation? CORRECT ANSWER-Chemical reaction (formaldehyde) and physical (heat or dessication) What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** CORRECT ANSWER-bacteria or mold What factor causes autolysis if tissue is not fixed? **** CORRECT ANSWER-enzyme attack What are four functions of fixatives? CORRECT ANSWER-1. kills tissue ...

Histotechnology Mid TermWhen is H&E QC done? - correct answers -Daily -Before any patient slides are stained What is H&E QC? - correct answers -Slides are checked manually for approval -Documented and logged for future inspections What is the meniscus? - correct answers -Bottom line of the curved surface of the liquid How many grams are needed to make 500mL of the 10% solution? - correct answers % desired:100 = g needed:volume desired -50g What causes tissue to shrink, dry, brit...

Neoadjuvant therapy does not increase survival when compared to adjuvant therapy. It only changes the timing of treatment and can change surgical options if the tumor is shrunk enough. If this occurs, the patient may only require a lumpectomy plus radiation therapy instead of needing a mastectomy - Correct answer-Main benefit of neoadjuvant chemotherapy (breast cancer patient) A comprehensive geriatric assessment (CGA) is a multidisciplinary evaluation to assess life expectancy and risk of...

Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive ...

Acetic acid CORRECT ANSWER-This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde CORRECT ANSWER-Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal CORRECT ANSWER-This fixative is the smallest ...

Alcian Blue demonstrates - Answer Acid mucins ATPase Enyme Histochemistry demonstrates - Answer ATPase in muscles Bielschowsky's Silver Technique demonatrates - Answer Dendrites, axons, neurofibrils Brown & Brenn gram stain demonstrates - Answer bacteria Congo red demonstrates - Answer amyloid Cresyl Echt Violet demonstrates - Answer Nissl substance Geimsa stain demonstrates - Answer mast cells or H. pylori Gomori Burtner demonstrates - Answer melani...

fixitive used for specimens - correct answers 10% neutral buffered Formalin Marking Margins - correct answers - to identify resection margins, if tumour too close to these margins they know they have to go in and cut more out - Guide to embedding What is used to clean grossing station - correct answers phenolic disenfectant or gluteroldehyde how long is specimens stored for - correct answers 1-3 months some legal cases kept indefinitly Why is fresh tissue not stored? - correct answ...

Histotechnology: A Self-Instructional Text, 4th edFor adequate fixation, the fixative volume should be at least ____ times greater than the tissue volume. A. Equal B. 5-10 C. 10-14 D. 15-20 - correct answers 15-20 This type of microscope is used for the identification of crystals as well as to make identification of amyloid A. Light microscope B. Polarizing microscope C. Phase-contrast microscope D. Darkfield microscope - correct answers Polarizing microscope For most routine H & E...

1. If there is venetian blind pattern in the section, a possible remedy is to: CORRECT ANSWER-Tighten all screws 2. To decrease the effect of static electricity: CORRECT ANSWER-ground microtome or breathe on it 3. To remedy crooked ribbon formation: CORRECT ANSWER-trim block edges parallel 4. If the specimen is mushy-appearing and sectioning is difficult: CORRECT ANSWER-a. melt block, reprocess & re-embed 6. A scratching noise during sectioning can be caused by: CORRECT ANSWER-tissue...