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[Show more]DNA was isolated from a patient and cut with HaeIII. The resulting fragments were 
separated by gel electrophoresis and transferred to a membrane. The fragments were 
allowed to hybridize to a probe, and the resulting band pattern was compared with that 
seen in another sample of DNA. Which of the f...
Preview 2 out of 5 pages
Add to cartDNA was isolated from a patient and cut with HaeIII. The resulting fragments were 
separated by gel electrophoresis and transferred to a membrane. The fragments were 
allowed to hybridize to a probe, and the resulting band pattern was compared with that 
seen in another sample of DNA. Which of the f...
What are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction 
enzymes 
2. Fragments are separated by gel electrophoresis 
3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), 
complementary to region of interest 
What is RFLP? - ️️- Restrictio...
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Add to cartWhat are the steps of a southern blot? - ️️1. DNA is isolated and cut with restriction 
enzymes 
2. Fragments are separated by gel electrophoresis 
3. DNA fragments are exposed to a labeled probe (complementary DNA or RNA), 
complementary to region of interest 
What is RFLP? - ️️- Restrictio...
Sequencing of the DNA region encoding 16S rRNA is performed to determine - 
️️the evolutionary and genetic relatedness of microorganisms and has driven 
changes in microorganism nomenclature 
Homologous extrinsic control - ️️target-derived control with a non-target-derived 
sequence insert; ...
Preview 2 out of 8 pages
Add to cartSequencing of the DNA region encoding 16S rRNA is performed to determine - 
️️the evolutionary and genetic relatedness of microorganisms and has driven 
changes in microorganism nomenclature 
Homologous extrinsic control - ️️target-derived control with a non-target-derived 
sequence insert; ...
Warfarin (Coumadin) - ️️CYP2C9 and VKORC1 - Vitamin K antagonist; 
metabolized primarily via oxidation in the liver by CYP2C9 and exerts its anticoagulant 
effect by inhibiting the protein vitamin epoxide reductase complex, subunit 1 (VKORC1) 
Chronic Myelogenous Leukemia (CML) - ️️t(9;22) a...
Preview 2 out of 5 pages
Add to cartWarfarin (Coumadin) - ️️CYP2C9 and VKORC1 - Vitamin K antagonist; 
metabolized primarily via oxidation in the liver by CYP2C9 and exerts its anticoagulant 
effect by inhibiting the protein vitamin epoxide reductase complex, subunit 1 (VKORC1) 
Chronic Myelogenous Leukemia (CML) - ️️t(9;22) a...
DNA polymerase delta - Answer-eukaryote: completes replication on the *lagging* 
strand; binds more tightly to DNA sliding clamp 
nucleotide and base excision repair 
Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping 
them from re-pairing 
topoisomerase 1 - Answer-Mak...
Preview 2 out of 6 pages
Add to cartDNA polymerase delta - Answer-eukaryote: completes replication on the *lagging* 
strand; binds more tightly to DNA sliding clamp 
nucleotide and base excision repair 
Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping 
them from re-pairing 
topoisomerase 1 - Answer-Mak...
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