Blotting - Study guides, Revision notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - ANSWER ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - ANSWER the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - ANSWER Hybridizes filter-bound DNA wi...

Ohio School of Phlebotomy CH. 3 & 4/ Q&A . Terms like: CBC - Answer: complete blood count Why is order of draw important? - Answer: To prevent contaminating later tubes with the additive from previous tubes. Evacuated tubes - Answer: Tubes with air removed to produce a vacuum Anticoagulant - Answer: prevents blood clotting

Gel Electrophoresis Questions and Answers Rated A+ What role does gel electrophoresis play in gene therapy research? Gel electrophoresis is used to analyze the delivery and integration of therapeutic genes into target cells, helping assess the effectiveness of gene therapy approaches. How can gel electrophoresis help identify pathogens in a clinical setting? By amplifying specific genetic markers of pathogens and separating them using gel electrophoresis, researchers can quickly ide...

CLP FINAL EXAM REVIEW QUESTIONS & ANSWERS 2024/2025 epistaxis - ANSWERSbleeding from the nose bandages - ANSWERSare cloth-like materials used to hold dressings in place. To secure splints, support and protect body parts. Bandages are not required to be sterile but they must be clean. dressing - ANSWERSare sterile coverings, patches or pads that is applied directly on the wound/injured area to control, absorb the bleeding, to promote healing and protect the are from further damage. ...

VTNE Practice Test (A) Which type of suture material is more likely to "wick" fluid along it therefore, should be be used in a contaminated environment? - Braided polyglactin 910 How many grams of sodium chloride should be dissolved in 1.5L of water to make a 12% solution? - 180g 1500 x 0.12 = 180 In treating hypovolemic shock in the canine, the most important form of therapy is to : - Administer IV fluids The lingual surface of the tooth is closest to the: - Tongue The st...

They can also be designed to only bind to one version of an allele - allele specific oligonucleotides (ASO). This can be used with PCR to report on whether samples contain certain mutations or polymorphisms. Southern Blotting - Answer Transfer of macromolecules onto a solid-phase membranous support such as nylon or nitrocellulose. This is done after gel electrophoresis and before hybridization in order to visualize results of these tests. Southern blotting refers to DNA blotting

basic stains CORRECT ANSWER methylene blue, crystal violet, safranin are all examples of what kind of stain? acidic stains CORRECT ANSWER nigrosin is an example of what kind of stain? 1. smear preparation 2. fixation (heat) 3. staining CORRECT ANSWER simple staining procedure bibulous paper CORRECT ANSWER a special absorbent paper used to dry slides; blotting paper 10x CORRECT ANSWER what objective lens should you always start with? 40x and 100x CORRECT ANSWER When focusing the ...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...