Southern blot - Study guides, Class notes & Summaries

Methylases - Answer adds methyl group to restriction sites on cell's own DNA (blocks cleavage) Silent mutation - Answer No change in amino acid sequence Nonsense mutation - Answer Introduction of stop codon Missense mutation - Answer Amino acid change at position Dispersive replication - Answer fragments of original DNA molecules are used as templates Reciprocal translocation - Answer exchange of one part of a chromosome for another (problems in meiosis) Re...

The pathophysiology of Glazmann thrombasthenia is: A. Deficiency of dense granules B. Deficiency of glycoprotein Ib/IX receptor C. Deficiency of glycoprotein IIb/IIIa receptor D. deficiency of microtubules - C. Deficiency of glycoprotein IIb/IIIa receptor Chagas disease is caused by: A. Trypanosoma cruzi B. Trypanosoma palladium C. Trypanosoma brucei D. None of the above - A. Trypanosoma cruzi What would a patient with a microcyctic/hypochromic anemia most likely have noted on their CB...

E. coli RNA Polymerase II - CORRECT ANSWER DNA template, product: mRNA RNA Polymerase I - CORRECT ANSWER DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - CORRECT ANSWER DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - CORRECT ANSWER DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - CORRECT ANSWER DNA template, product: mRNA Mam...

DNA was isolated from a patient and cut with HaeIII. The resulting fragments were separated by gel electrophoresis and transferred to a membrane. The fragments were allowed to hybridize to a probe, and the resulting band pattern was compared with that seen in another sample of DNA. Which of the following methods is described? a. RFLP by southern blot b. VNTR by PCR c. SNP by sequencing d. STR by PCR - ️️RFLP by southern blot Which of the following is used as a marker of gender? a. D...

Predominant form of DNA -Correct Answer Right-handed B DNA and histone interaction is due to what type of bond? -Correct Answer Ionic What stains to the minor groove of DNA duplex? -Correct Answer SYBR Green What is the most abundant type of RNA? -Correct Answer Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? -Correct Answer Genome and mRNA What increases half-life of mRNA? -Correct Answer 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active?...

Which of the following reactions is commonly used to replenish intermediates of the citric acid cycle? correct answer: glutamate --> ketoglutarate Which of the following statements is true regarding the activity of the ribonuceltide reductase? correct answer: The enzyme is required to convert nucleotides from the ribose form to the deoxyribose form required for DNA synthesis A Southern blot with a probe (denoted as XXXX in the figure) to DNA isolated from an individual who has both a ...

What is the most important factor in a successful PCR reaction? - Well designed primers What can the theory of primer design also be applied to? - The use of oligonucleotides in other techniques that utilize annealing or formations of duplexes of nucleotides (probes in Southern blot, Northern Blot, ect.) What variables influence the success of a PCR reaction? - - quality of template DNA - cycling conditions (melting temp., duration) - specificity and stability of the PCR primers What ...

vMCAT Exam Questions and Answers 100% CorrectMCAT Exam Questions and Answers 100% CorrectMCAT Exam Questions and Answers 100% CorrectMCAT Exam Questions and Answers 100% CorrectProducts of the Pentose Phosphate Pathway - ANSWER - NADPH, ribose- 5 - phosphate , Glycolytic intermediates reaction of G-6-phosphate to ribulose-5- phosphate is.. - ANSWER - oxidation of G6P where is the pentose phosphate pathway likely to happen - ANSWER - adipose tissue Southern Blot - ANSWER - used to analyz...

If you stepped on a rusty nail, which bacteria would most likely be the cause? CORRECT ANSWER Tetanus, botulism Put these events in correct order for humoral immunity 1. clonal selection 2. formation of memory B cells 3. B cell activation 4. formation of plasma B cells 5. immunological memory CORRECT ANSWER 3, 1, 4, 2, 5 DiGeorge Syndrom causes... CORRECT ANSWER T cell deficiency and hypocalcemia What age do you have the best immunity CORRECT ANSWER 10 The picture for ELISA t...

MICROBIOLOGY EXAM 2 (LECTURE 7-13) EXAM REVIEW QUESTIONS AND ANSWERS, GRADED A+/ VERIFIED. Diagnostic method may require a longer time to reach a diagnosis due to the culturing of microbes Phenotypic Diagnostic method that usually does not requires culturing techniques Genotypic Diagnostic techniques that may or may not require culturing but do take advantage of antigen-antibody specificity Immunological Examples of phenotypic method microscopic macroscopic physiological/biochemica...