100% tevredenheidsgarantie Direct beschikbaar na betaling Zowel online als in PDF Je zit nergens aan vast
logo-home
Summary Mammalian cell biology €5,49   In winkelwagen

Samenvatting

Summary Mammalian cell biology

 15 keer bekeken  0 keer verkocht

Mammalian cell biology

Voorbeeld 3 van de 18  pagina's

  • 15 april 2022
  • 18
  • 2021/2022
  • Samenvatting
Alle documenten voor dit vak (1)
avatar-seller
annerixtvanderwal
Mammalian cell biology lecture 1 31-8
Species: closely related organisms that are similar and capable of interbreeding and
producing fertile offspring.

Realm -> domains
Lumen -> vaatholte

Metabolites dissolves in water
Lipids dissolves in fat

Cytoplasm -> all contents of a cell exept cell membrane and nucleus
Cytosol -> Cytoplasm without organelles

Mitosis is symmetric cell dividsion and Meiosis is asymmetric cell division

Transcription
Translation
DNA -> AGCT(Thymine) Double-stranded
RNA -> AGCU(Uracil) Single-stranded

RNA-splicing introns and exons (introns are removed from protein-coding exons)
Isoforms are different protiens that derive form the same gene.
Triplet -> codon

Stop and start codon
9 essential aminoacids
Primary-, Secundary- tertiary- and Quatenary structure
Virus are not living organisms, it is a membrane that is packed with proteins that hijack host
ribosomes and proteins for replications. That way they can replicate quickly.

Peptide bond/amide bond formed by de-hydrolysis(condensation)
Cis- & transbonds
Alpha-helix & Beta-sheets
Phosphorylation activates or deactivates protiens
Ubiquitinations
-small protein (76 amino acids)
- multiple conjucated to lysine of target protein
- marks protein for: degradation by proteasome/ endocytosis

Proteasome is a large barrel-shaped protein structure in cytosol that degrades protein.
Transporters
Channels: Passive transport (holes)
Secondary transporters: use gradient of other molecules (diffusion and osmosis)
ATP-binding cassette (ABC) transporter: ATP-couples active transport

Sugar metabolism consists of Glycolysis(cytosol) and Oxidative respiration(mitochondria)


1

,CO2 cycle by photosynthesis and respiration, what photosynthesis creates is used with
respiration.

Metabolism: catabolic (produces energy) and anabolic (consumes energy)
ATP is the main energy storage of cells.

Mammalian cell biology lecture 2 7-9

Rough ER has punctuated structured membrane. The ribosomes are on the membrane of the
ER.
Co- translational insertion in rough ER.
SRP binds to the signal sequence and stops the process of translation. It pulls the ribosome
to the Translocon. It binds to the ER. There the signal sequence remains in the ER and then
the translation continues.

Binding immunoglobulin protein (BiP) helps protein translocation and folding. BiP prevents
premature folding.

Trans-membrane proteins
- Type 1 (N-terminus in ER lumen)
- Type 2 (C-terminus in ER lumen)
- Type 3 (N-terminal (signal-anchor) in membrane)
- Tail-anchored (C-terminus in membrane)
- Type 4 has multiple transmembrane segments

N-terminus has a primary aiming the protein starts with an N-terminus (NH3+) in a neutral
enviourment.
C-terminus has a carboxylic group the protein stops with a C-terminus (COO-)

Hydrophobic inside the membrane.


Stop-transfer anchor (STA)
- Hydrophobic trans-membrane segment
- Prevents translocation over membrane
- Lateral opening of Sex61 translocon
- Will become transmembrane segment
Signal anchor (SA)
- Translocation after synthesis
- Targeting via SRP
- Positive charge determines orientation
- Are not cleaved of

Type 2 has no a signal sequence and there is no cleavage of the signal.
Type 1 membrane proteins have a signal sequence type 3 does not.

Positive inside(cytosol) rule

2

, - Charge of residues next to signal anchor determines orientation
- Largest positive charge in cytosol.
Signal anchors are similar to signal sequences but are not cleaved of.

Type 4 is a combination of type 1,2 & 3. Combination of STA and SA. Has multiple
transmembrane regiouns
SA is made before it is inserted into the membrane

If the chargers are at the c terminus it is type 3 if it is at the n terminus it is type 2.

Polar is hydrophilic.
GPI-anchored are always type 1 proteins.

Post-translational insertion into the mitochondria. Some proteins are transcribed and
translated within mitochondria. They have their own (small) chromosome, this codes for 13
proteins.

Chaprerone Hsc70 binds in a ATP-dependent manner to keep the protein unfolded to get
into the mitochondria.

Tom: transporter of the outer membrane
Tim: transporter of the inner membrane

1. Dissociation of cytosolic Hsc70 consumes ATP
2. Translocation of cationic signal sequence through TIM mediated by membrane
potential
3. Dissociation of matrix Hsc70 consumes ATP

Post-translational insertion into nucleus meaning the protein is already fully formed. Into the
nucleus the protein is folded which is different to the mitochondria.

Karyopherins
Small-GTPase are like molecular switches which can be on/off switch

Ran-GDP state is cytosol by Ran-GAP
Ran -GTP state in nucleus by Ran-GEF

Mammalian cell biology lecture 3 14-9

Major groove & minor groove
10 base pairs make one helix part = 3,4 nm = 1 turn
DNA is righthanded tumb points in the direct of movement

Sugar piece and phosphate (5 prime end 5’)
- C-G 3 hydrogen bonds
- A-T 2 hydrogen bonds


3

Voordelen van het kopen van samenvattingen bij Stuvia op een rij:

Verzekerd van kwaliteit door reviews

Verzekerd van kwaliteit door reviews

Stuvia-klanten hebben meer dan 700.000 samenvattingen beoordeeld. Zo weet je zeker dat je de beste documenten koopt!

Snel en makkelijk kopen

Snel en makkelijk kopen

Je betaalt supersnel en eenmalig met iDeal, creditcard of Stuvia-tegoed voor de samenvatting. Zonder lidmaatschap.

Focus op de essentie

Focus op de essentie

Samenvattingen worden geschreven voor en door anderen. Daarom zijn de samenvattingen altijd betrouwbaar en actueel. Zo kom je snel tot de kern!

Veelgestelde vragen

Wat krijg ik als ik dit document koop?

Je krijgt een PDF, die direct beschikbaar is na je aankoop. Het gekochte document is altijd, overal en oneindig toegankelijk via je profiel.

Tevredenheidsgarantie: hoe werkt dat?

Onze tevredenheidsgarantie zorgt ervoor dat je altijd een studiedocument vindt dat goed bij je past. Je vult een formulier in en onze klantenservice regelt de rest.

Van wie koop ik deze samenvatting?

Stuvia is een marktplaats, je koop dit document dus niet van ons, maar van verkoper annerixtvanderwal. Stuvia faciliteert de betaling aan de verkoper.

Zit ik meteen vast aan een abonnement?

Nee, je koopt alleen deze samenvatting voor €5,49. Je zit daarna nergens aan vast.

Is Stuvia te vertrouwen?

4,6 sterren op Google & Trustpilot (+1000 reviews)

Afgelopen 30 dagen zijn er 64438 samenvattingen verkocht

Opgericht in 2010, al 14 jaar dé plek om samenvattingen te kopen

Start met verkopen
€5,49
  • (0)
  Kopen