Samenvatting
Summary Mammalian cell biology
- Instelling
- Rijksuniversiteit Groningen (RuG)
Mammalian cell biology
[Meer zien]Voorbeeld 3 van de 18 pagina's
Voorbeeld 3 van de 18 pagina's
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Voorbeeld van de inhoud
Mammalian cell biology lecture 1 31-8Species: closely related organisms that are similar and capable of interbreeding and
producing fertile offspring.
Realm -> domains
Lumen -> vaatholte
Metabolites dissolves in water
Lipids dissolves in fat
Cytoplasm -> all contents of a cell exept cell membrane and nucleus
Cytosol -> Cytoplasm without organelles
Mitosis is symmetric cell dividsion and Meiosis is asymmetric cell division
Transcription
Translation
DNA -> AGCT(Thymine) Double-stranded
RNA -> AGCU(Uracil) Single-stranded
RNA-splicing introns and exons (introns are removed from protein-coding exons)
Isoforms are different protiens that derive form the same gene.
Triplet -> codon
Stop and start codon
9 essential aminoacids
Primary-, Secundary- tertiary- and Quatenary structure
Virus are not living organisms, it is a membrane that is packed with proteins that hijack host
ribosomes and proteins for replications. That way they can replicate quickly.
Peptide bond/amide bond formed by de-hydrolysis(condensation)
Cis- & transbonds
Alpha-helix & Beta-sheets
Phosphorylation activates or deactivates protiens
Ubiquitinations
-small protein (76 amino acids)
- multiple conjucated to lysine of target protein
- marks protein for: degradation by proteasome/ endocytosis
Proteasome is a large barrel-shaped protein structure in cytosol that degrades protein.
Transporters
Channels: Passive transport (holes)
Secondary transporters: use gradient of other molecules (diffusion and osmosis)
ATP-binding cassette (ABC) transporter: ATP-couples active transport
Sugar metabolism consists of Glycolysis(cytosol) and Oxidative respiration(mitochondria)
1
,CO2 cycle by photosynthesis and respiration, what photosynthesis creates is used with
respiration.
Metabolism: catabolic (produces energy) and anabolic (consumes energy)
ATP is the main energy storage of cells.
Mammalian cell biology lecture 2 7-9
Rough ER has punctuated structured membrane. The ribosomes are on the membrane of the
ER.
Co- translational insertion in rough ER.
SRP binds to the signal sequence and stops the process of translation. It pulls the ribosome
to the Translocon. It binds to the ER. There the signal sequence remains in the ER and then
the translation continues.
Binding immunoglobulin protein (BiP) helps protein translocation and folding. BiP prevents
premature folding.
Trans-membrane proteins
- Type 1 (N-terminus in ER lumen)
- Type 2 (C-terminus in ER lumen)
- Type 3 (N-terminal (signal-anchor) in membrane)
- Tail-anchored (C-terminus in membrane)
- Type 4 has multiple transmembrane segments
N-terminus has a primary aiming the protein starts with an N-terminus (NH3+) in a neutral
enviourment.
C-terminus has a carboxylic group the protein stops with a C-terminus (COO-)
Hydrophobic inside the membrane.
Stop-transfer anchor (STA)
- Hydrophobic trans-membrane segment
- Prevents translocation over membrane
- Lateral opening of Sex61 translocon
- Will become transmembrane segment
Signal anchor (SA)
- Translocation after synthesis
- Targeting via SRP
- Positive charge determines orientation
- Are not cleaved of
Type 2 has no a signal sequence and there is no cleavage of the signal.
Type 1 membrane proteins have a signal sequence type 3 does not.
Positive inside(cytosol) rule
2
, - Charge of residues next to signal anchor determines orientation
- Largest positive charge in cytosol.
Signal anchors are similar to signal sequences but are not cleaved of.
Type 4 is a combination of type 1,2 & 3. Combination of STA and SA. Has multiple
transmembrane regiouns
SA is made before it is inserted into the membrane
If the chargers are at the c terminus it is type 3 if it is at the n terminus it is type 2.
Polar is hydrophilic.
GPI-anchored are always type 1 proteins.
Post-translational insertion into the mitochondria. Some proteins are transcribed and
translated within mitochondria. They have their own (small) chromosome, this codes for 13
proteins.
Chaprerone Hsc70 binds in a ATP-dependent manner to keep the protein unfolded to get
into the mitochondria.
Tom: transporter of the outer membrane
Tim: transporter of the inner membrane
1. Dissociation of cytosolic Hsc70 consumes ATP
2. Translocation of cationic signal sequence through TIM mediated by membrane
potential
3. Dissociation of matrix Hsc70 consumes ATP
Post-translational insertion into nucleus meaning the protein is already fully formed. Into the
nucleus the protein is folded which is different to the mitochondria.
Karyopherins
Small-GTPase are like molecular switches which can be on/off switch
Ran-GDP state is cytosol by Ran-GAP
Ran -GTP state in nucleus by Ran-GEF
Mammalian cell biology lecture 3 14-9
Major groove & minor groove
10 base pairs make one helix part = 3,4 nm = 1 turn
DNA is righthanded tumb points in the direct of movement
Sugar piece and phosphate (5 prime end 5’)
- C-G 3 hydrogen bonds
- A-T 2 hydrogen bonds
3
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