Biosketch is an account of the life and activities of the individual. It includes three key elements of the person's life: personal details, professional details, and social details. The content must be written in third person as it is written by someone else. The summary must be in chronological o...
Question paper - 30 marks
Q1. Make a temporary stained preparation of T.S of Sunflower stem. (4)
OR
Q1. Make a temporary stained squash of onion root tip to show various stages of mitosis.
Sketch and label any one stage of mitosis. (4)
Q2. Perform the biochemical test for starch / sugar / protein / fats (3)
Q3. Prepare a temporary stained slide of squamous epithelium. (3)
Q4. Comment on the spots (A to J) as per instruction. (10)
A. Plant specimens (Oscillatoria / Agaricus/ Sargassum / Nephrolepis)
B. Animal specimens (Hydra / Snail / Star fish / Ascaris)
C. Plant anatomy (sunflower root / maize root)
D. Plant modifications (Fusiform root / Parasitic roots / Rhizome / Phylloclade)
E. Animal tissues (T.S of bone / striated muscles)
F. Physiological experiment (aerobic / anaerobic respiration)
G. Cockroach external morphology (head/thorax/abdomen) or digestive system
(salivary gland / crop)
H. Cockroach mouth parts (mandible)
I. Histology of digestive organs (T.S of pancreas / T.S of Small intestine)
J. Bones (Humerus/ Femur)
Q5. Project (5)
Q6. Viva (2)
Q7. Certified journal (3)
, Q1. Make a temporary, stained preparation of T.S of sunflower stem. (4 marks)
Slide preparation – 3 marks (NOT TO WRITE ON THE ANSWER SHEET)
o Take 8 to 10 thin sections of the sunflower stem given and put them in a watch glass containing water.
o Select and transfer one thinnest section in another watch glass containing dilute safranin and let it stain for
1 -2 mins.
o Take a clean slide and put a drop of glycerine in the centre of the slide.
o With the help of a brush transfer well stained section on the slide. Place a coverslip avoiding any air bubbles.
o Blot excess of glycerine from the sides of the coverslip. Observe the slide under 10X objective of microscope
Draw labelled diagram of the examiner’s choice out of the following on the answer sheet: (1mark)
Parenchyma Collenchyma
Probable Viva Voce
1. Why is Safranin stain used? (It highlights dead tissues like sclerenchyma and xylem vessels)
2. How are the vascular bundles arranged in this stem? - (In a ring)
3. Describe the vascular bundles of sunflower stem. (Conjoint, Collateral & Open)
4. Which kind of cells is the hypodermis made up of? (Collenchyma)
5. Are trichomes present? If yes, are they uni or multicellular? (Yes, Multicellular)
6. Is sunflower stem dicot or monocot? Why? Dicot (because vascular bundles are open, in a ring and not
scattered as in monocot)
Q 1. Mitosis in onion root tip (4 marks)
OR
Squash preparation – 2 marks (NOT TO WRITE ON THE ANSWER SHEET)
o Take a root tip in watch glass and wash it with water two times. Drain off the water
o Add 1 N HCl to the watch glass and warm it over the spirit lamp.
o Remove from the flame and let it cool for some time.
o Remove the HCl and wash the root tip two times with water.
o Take the root tip on a clean slide.
o Cut and keep the milky white portion of the root tip on the centre of the
slide, discard the remaining part.
o Put a drop of Acetocarmine stain.
o Let the root tip remain in acetocarmine for 20-25 minutes.
o After 20-25 mins put coverslip on it and squash it.
o Observe first under 10x (low power) and then under 45x (high power).
Identification of any stage of mitosis under the microscope - 1 m
Probable Viva Voce
1. What is mitosis?
2. Why is it called as an equational division? (distributes equal no. of chromosomes in the daughter cells)
3. Why is the root tip treated with 1N HCl? (to dissolve middle lamella)
4. Why is root tip selected as a raw material? (Because it has meristematic tissue)
5. Why is Acetocarmine stain used? (It is a nuclear stain so it stains chromosomes)
6. State any two characteristic features of Prophase, Metaphase, Anaphase, Telophase
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