UNE Biochemistry Midterm Study Bank Questions
With Complete Solutions
Succinate is a substrate for which complex in the electron
transport chain?
a.) Complex 1 (NADH-1 oxidoreductase)
b.) Complex IV (Cyt c oxidase)
c.) Complex V (ATP synthase)
d.) complex II (Succinate-Q reductase) correct answer:
Complex II (succinate-Q reductase) (D)
Which of the following would be consistent with an exergonic
reaction?
a.) ∆S would be negative
b.) ∆G would be negative
c.) ∆S would be positive
d.)∆G would be positive correct answer: ∆G would be negative
(B)
Which of the following describes the malate-aspartate shuttle
system?
,a.) Carries NADH from the cytoplasm directly into the
mitochondria
b.) Generates 2.5 molecules of ATP in mitochondria per each
NADH from glycolysis
c.) Moves NADH from the mitochondria to the cytoplasm
d.) Relies on malate dehydrogenase in the inner mitochondrial
membrane
e.) Requires reduction of pyruvate to lactate for oxidizing
NADH correct answer: Generates 2.5 molecules of ATP in
mitochondria per each NADH from glycolysis (B)
ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of
the following components of the molecule?
a.) In the high energy phosphate bonds
b.) In the adenine base
c.) In the D-ribose group correct answer: In the high energy
phosphate bonds (A)
Dinitrophenol is an uncoupler of the electron transport chain.
The addition of dinitrophenol will have which of the following
impacts?
,a.) Decrease the pH of the intermembrane space
b.) Increase oxygen consumption
c.) Decrease oxidation of NADH
d.) Increase ATP production correct answer: Increase oxygen
consumption (B)
In the malate aspartate shuttle, glutamate is converted to
_______ in the mitochondria while aspartate is converted to
_______ in the cytosol.
a.) Oxaloacetate; malate
b.) alpha-ketoglutarate; oxaloacetate
c.) Oxaloacetate; glutamate
d.) Aspartate; alpha-ketoglutarate
e.) Malate; oxaloacetate correct answer: alpha-ketoglutarate;
oxaloacetate (B)
The glycerophosphate shuttle is a major shuttle of most tissues.
Which of the following best describes the transfer of electrons
by this shuttle?
a.) Electrons are directly transferred from glycerol-3-phosphate
to NAD containing glycerol phosphate dehydrogenase
, b.) Electrons are directly transferred from glycerol-3-phosphate
to an FAD containing glycerol phosphate dehydrogenase
c.) Electrons are directly transferred from DHAP
(dihydroxyacetone phosphate) to an FAD containing glycerol
phosphate dehydrogenase
d.) Electrons are directly transferred from glycerol-3-phosphate
and DHAP correct answer: Electrons are directly transferred
from glycerol-3-phosphate to FAD containing glycerol
phosphate dehydrogenase (B)
Heat can be generated by brown adipose tissue in hibernating
mammals. Which of the following is most likely the mechanism
by which this occurs?
a.) Increased ATP production by ATP synthase
b.) Expression of a protein that uncouples the ETC
c.) An increased pH gradient across the inner mitochondrial
membrane
d.) A decrease in oxygen consumption correct answer:
Expression of a protein that uncouples the ETC (B)
Allosteric activators of hemoglobin will increase binding
through what mechanism? correct answer: Binding the protein,
and keeping it in the "R" conformation
With Complete Solutions
Succinate is a substrate for which complex in the electron
transport chain?
a.) Complex 1 (NADH-1 oxidoreductase)
b.) Complex IV (Cyt c oxidase)
c.) Complex V (ATP synthase)
d.) complex II (Succinate-Q reductase) correct answer:
Complex II (succinate-Q reductase) (D)
Which of the following would be consistent with an exergonic
reaction?
a.) ∆S would be negative
b.) ∆G would be negative
c.) ∆S would be positive
d.)∆G would be positive correct answer: ∆G would be negative
(B)
Which of the following describes the malate-aspartate shuttle
system?
,a.) Carries NADH from the cytoplasm directly into the
mitochondria
b.) Generates 2.5 molecules of ATP in mitochondria per each
NADH from glycolysis
c.) Moves NADH from the mitochondria to the cytoplasm
d.) Relies on malate dehydrogenase in the inner mitochondrial
membrane
e.) Requires reduction of pyruvate to lactate for oxidizing
NADH correct answer: Generates 2.5 molecules of ATP in
mitochondria per each NADH from glycolysis (B)
ATP is a high energy molecule that is often hydrolyzed during
reactions. The energy within ATP is stored primarily in which of
the following components of the molecule?
a.) In the high energy phosphate bonds
b.) In the adenine base
c.) In the D-ribose group correct answer: In the high energy
phosphate bonds (A)
Dinitrophenol is an uncoupler of the electron transport chain.
The addition of dinitrophenol will have which of the following
impacts?
,a.) Decrease the pH of the intermembrane space
b.) Increase oxygen consumption
c.) Decrease oxidation of NADH
d.) Increase ATP production correct answer: Increase oxygen
consumption (B)
In the malate aspartate shuttle, glutamate is converted to
_______ in the mitochondria while aspartate is converted to
_______ in the cytosol.
a.) Oxaloacetate; malate
b.) alpha-ketoglutarate; oxaloacetate
c.) Oxaloacetate; glutamate
d.) Aspartate; alpha-ketoglutarate
e.) Malate; oxaloacetate correct answer: alpha-ketoglutarate;
oxaloacetate (B)
The glycerophosphate shuttle is a major shuttle of most tissues.
Which of the following best describes the transfer of electrons
by this shuttle?
a.) Electrons are directly transferred from glycerol-3-phosphate
to NAD containing glycerol phosphate dehydrogenase
, b.) Electrons are directly transferred from glycerol-3-phosphate
to an FAD containing glycerol phosphate dehydrogenase
c.) Electrons are directly transferred from DHAP
(dihydroxyacetone phosphate) to an FAD containing glycerol
phosphate dehydrogenase
d.) Electrons are directly transferred from glycerol-3-phosphate
and DHAP correct answer: Electrons are directly transferred
from glycerol-3-phosphate to FAD containing glycerol
phosphate dehydrogenase (B)
Heat can be generated by brown adipose tissue in hibernating
mammals. Which of the following is most likely the mechanism
by which this occurs?
a.) Increased ATP production by ATP synthase
b.) Expression of a protein that uncouples the ETC
c.) An increased pH gradient across the inner mitochondrial
membrane
d.) A decrease in oxygen consumption correct answer:
Expression of a protein that uncouples the ETC (B)
Allosteric activators of hemoglobin will increase binding
through what mechanism? correct answer: Binding the protein,
and keeping it in the "R" conformation
