MICROBIOLOGY PORTAGE - MODULE 4 MICROBIAL GROWTH AND CONTROL EXAM QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED GRADED A+
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MICROBIOLOGY PORTAGE - MODULE 4 MICROBIAL GROWTH AND CONTROL EXAM QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED GRADED A+
Expanding a given microbial population allows researchers to do what?
to detect and assess the microorganism more easily
What is Growth Media?
Growth media is a liquid or sol...
MICROBIOLOGY PORTAGE - MODULE 4 MICROBIAL GROWTH AND
CONTROL EXAM QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED
GRADED A+
Expanding a given microbial population allows researchers to do what?
to detect and assess the microorganism more easily
What is Growth Media?
Growth media is a liquid or solid formulated to support microbial growth.
What does Growth Media contain?
The media contains essential nutrients to provide the microbe with a source of
carbon/energy. The nutrient composition often includes a rich source of sugars, amino
acids and vitamins.
What else can Growth Media also be called?
Such media can also be referred to as a nutrient broth and is commonly used to grow
microbes in a suspension.
Describe LB media
Perhaps the most common nutrient broth in the lab, LB media (lysogeny broth) is a
nutritionally rich liquid known for its ability to grow a vast array of microbes.
What is Selective Media?
Selective media allows for only the growth of certain microbes and, by extension,
restricts the growth of all others.
How can Selective Media be accomplished?
,Such an approach can be accomplished in a variety of ways including: using limiting
amounts of nutrients, varying degrees of pH (being either very acidic or very basic
media), or various chemical additives that limit unwanted microbial growth (i.e.)
antibiotics.
Describe how Selective Media works with Gram-Positive versus Gram-Negative
bacteria.
If a plate is selective for Gram-Negative then it will only grow Gram-Negative bacteria
on it. It will not grow Gram-Positive bacteria on it.
What is Differential Media?
Differential media distinguishes between two, often related, microbes.
What is one way that a Differential Media can distinguish between Escherichia
coli and Salmonella?
They are both Gram negative but can be distinguished by the presence (or absence) of
lactose fermentation.
If grown on the same differential media, E coli ferments lactose and turns the culture
red.
In contrast, Salmonella does not ferment lactose and the culture remains white/tan in
color.
(This above example is probably on MacConkey agar)
What is a fastidious microorganism?
, an organism with complex growth requirements such that if absent it will not grow
What are Enriched Medias used for?
Enriched media is used to grow fastidious microorganisms
Enriched Medias contain what?
enriched medias contain the essential nutrients required for the growth of this subset of
microorganisms
Can a plate be a combination of selective, differential and enriched?
Yes
Growth Media comes in what 2 forms?
liquid and solid
What is Solid Growth Media?
Solid growth media, often contained within a sterile petri dish, is in its simplest form
liquid growth media that has had a hardening agent added.
What makes agar?
The addition of a polysaccharide derived from seaweed (algae) extract added to the
liquid growth media creates a solid medium called agar.
What is a colony?
As they grow, microbes form colonies that take on the appearance of individual isolated
dots.
Why does dehydrated agar (and other necessary components) get mixed with
water and heated to a high temperature?
to dissolve the agar into solution. The high temperature also ensures sterility as it will kill
most, if not all, foreign microbes.
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