The liver cell is one of the few types of cell of the body which carries important function. It is involved in the process of metabolism of proteins, carbohydrates and the fats. It is of prime importance in bilirubin metabolism and secretes conjugated bilirubin into the bile. It has a role in the d...
EVALUATION OF HEPATOPROTECTIVE
ACTIVITY OF MARKETED HERBAL DRUG
PREPARATION
ABSTRACT
The aim of the present study is to evaluate the protective effect of marketed herbal preparation (Duliv) against carbon
tetrachloride (CCl4) induced liver damage in Albino rats. Administration with Duliv Tablet (100mg/kg p.o.)and Duliv Syrup
0.64 ml/100 gm for 15 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage,
SGPT,SGOT, albumin,protein , cholesterol and alkaline phosphatase.The histopathological studies in the liver of rats
also supported that Duliv tablet and Duliv syrup markedly reduced the toxicity of CCl4 and preserved the histoarchitecture
of the liver tissue to near normal. Thus, the results suggest that Duliv preparation acts as a potent hepatoprotective
agent against CCl4 induced hepatotoxicity in rats.
Keywords: Duliv tablet, Carbon tetrachloride, SGPT, SGOT, Hepatoprotective.
INTRODUCTION even then the liver is in a position to carry on the
normal physiological functions without any external
The liver cell is one of the few types of cell of the body clinical signs detectable by the physician.2
which carries important function. It is involved in the Liver damage is not easy to detect early by an existing
process of metabolism of proteins, carbohydrates and testing methods. This is one of the most important
the fats. It is of prime importance in bilirubin obstacles in the research progress regarding liver.
metabolism and secretes conjugated bilirubin into the Secondly the response of the liver to any damaging
bile. It has a role in the detoxification of harmful factor does not vary. It always culminates into
endogenous toxins e.g. Ammonia and purines and the Cirrhosis or the Fibrosis which replaces the injured or
exogenous toxins such as various drugs. dead hepatocytes. This makes difficult to assess the
Liver disease may be considered as being either diffuse effect of the drug. A number of etiological factors thus
or focal. In diffuse or generalized liver disease e.g. ultimately culminate into end stage liver disease that
hepatitis or bile duct obstruction, impaired liver function is known as cirrhosis.
is usually an early manifestation and may result in Right from alcohol to drugs and viruses virtually many
Jaundice. Acute liver failure, fluid retention, amoebic things can damage the liver cells if the challenge
abscess or there may be multiple lesions. E.g. metastatic continues to attack the organ. Different toxic
tumour in this focal disease, interference with overall substances from viruses and bacteria, to the artificial
liver functions may be in a late complication.1 food, flavours, colours, preservatives and antibiotics,
The problem of liver damage in both acute as well as these are the number of substances capable of
chronic, continue to trouble the medical profession producing the acute as well as chronic liver damage.
throughout the world as even today we have very little The present treatment of the liver diseases according
knowledge about the exact mode of functioning of the to modern allopathic medicines is not very satisfactory
liver. We have lot of knowledge about the enzymes which which advocates only, rest and fat free diet and leaves
the liver holds in its parenchyma and many chemical everything to the liver to regenerate by itself. There
reactions going on in this organ but with all is no specific therapy, in fact for the prevention as well
as the cure of the acute and chronic liver diseases.
*Corresponding author: Hence there is an ever increasing need for an agent
Dr. Jangme C.M.
which could protect against the liver damage. Up till
MaharashtraCollege of Pharmacy,
Nilanga, Dist: Latur, Maharashtra, India now, no correct medicine has been we available from
synthetic drugs. Hence the reliance is given only on
this knowledge we cannot accurately predict either Ayurveda Medicine for the treatment of liver diseases
the recovery or the damage in the liver. This is because and disorder.3In this way the liver preparations
in the liver the damage and the repair is going on side mentioned in Ayurvedic treatise or authoritative books
by side and the organ has great reserves. So that even have a special importance in medicines. It would be a
if seven parts out of the eight of the liver is damaged valued interest to find out the efficacy of this liver
8
, preparation in different, induced liver diseases and 1. Measurment of pentobarbitone sleeping time. B)
disorder, wherever possible in animals models. In Morphological Paramenters.
present study, evaluation of hepatoprotective 1. Weight of Liver.
preparation mentioned in Ayurveda is attempted on 2. Volume of Liver.
modern lines to test its claimed effects in liver disorders. C) Biochemical Parameters:
Plan of Work: 1. Serum glutamate oxaloacetate Transaminase (SGOT)
Hepatocellular disease is known to be associated with 2. Serum glutamate pyruvate Transaminase (SGPT)
impaired hepatic drug metabolising capacity and 3. Alkaline Phosphatase.
impaired activity of hepatic enzymes. Liver diseases are 4. Serum bilirubin
of different aetiology and till do day the best remedy is 5. Cholesterol
from natural drugs only. Ayurvedic books have prescribed 6. Total Proteins
as number of formulations in the treatment of liver 7. Albumin
diseases. 8. Globulin.
A number of indigenous formulations have been claimed D) Histological changes Such as:
to possess hepatoprotective activity. A few of the have 1. Fatty degeneration 2.
also been studied in experimental models. It will be of Focci of Necrosis
great value if all such formulations are biologically 3. Congestions.
Table 1: Active ingredient with their quantity of both formulations
Each Sugar coated Duliv Syrup
Common name Biological Name tablet contains Each ml contains.
(Formulation A) (Formulation B)
Sharpunkha TephrosiaPurpurea 150mg 200 mg
BhuiAvla Phyllanthusnuriri 40mg 50 mg
Maka Eclipta alba 40mg 50 mg
Sunth ZingiberOfficinale 10mg 25 mg
Tulas Ocimum Sanctum 10mg 25 mg
Hirda TerminaliaChebula 30mg 50 mg
Gulvel Tenosporacordifolia 20 mg 25 mg
Kalmegh AndrographisPeniculata 50 mg 20 mg
Punarnava Boerhaaviadiffussa 50 mg 100 mg
assessed on animal models, wherever possible, and also E) The statistical Significance.
by clinical trials. This will be revealing the utility of The overall objectives of these methods to be applied is
these formulations in liver diseases of varying aetiology. to prepared a monograph for the above mentioned
“DULIV” Is an indigenous herbal formulation which is hepatoprotective formulation.
claimed to be hepatoprotective. It contains nine
indigenous herbs which have been mentioned in MATERIALS AND METHODS
Ayurveda as hepatoprotective. “DULIV”an indigenous herbal formulation contains nine
In the present investigation effect of “DULIV TABLET” indigenous herbs which have been mentioned in
and “DULIV SYRUP”prepared by incorporating well Ayurveda as hepatoprotective. However no scientific
known indigenous plants was studied in Carbon study of the formulation prepared from these plants has
tetrachloride induced liver damage in Albino Rats. The been reported.
entire experimentation was carried out in the following The present study has been undertaken to evaluate the
way: role of DULIV as a function of time on the toxic effect
1. Procurement of drugs of the above mentioned induced by CCL4challenge on Serum enzyme. DULIV was
formulation. obtained as: Formulation ‘A’( Duliv Tablet)
2. Determination of safe dose of these formulations by Formulation ‘B’( Duliv Surup)
using Albino mice. Tablet was crushed and the powder (100mg/kg p.o. daily
3. Induction of hepatotoxicity in albino rats by using for 15days) was given as freshly prepared suspension in
CCL4. gum acacia. Control animals received only the vehicle.
4. Treatment of toxicity by administering formulation The literature survey has revealed that the
separately in different groups of albino rats. entireingredients in this formulation are individually
5. Assessment of hepatoprotective effect by using the having hepatoprotective activity. Determination of Safe
parameters as given below and subsequent Dose (Toxicity studies): Duliv preparation was studied
comparison. for its hepatoprotective activity. Prior to determine this
A) Functional Paramter.
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