Chap 11 Questions and Answers
An immunoassay is performed in the following manner to look for an antigen (Ag) in the patient serum: Patient serum + labeled Ag + known antibody (Ab) are added together and incubated. Unbound material is washed off. The amount of labeled Ag is then measured. What ass...
best describes competitive binding assays a limite
identity the characteristics associated with non c
best describes homogenous assays a washing step is
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Chap 11 Questions and Answers
An immunoassay is performed in the following manner to look for an antigen (Ag) in the patient serum: Patient serum + labeled Ag + known antibody (Ab) are added together and incubated. Unbound material is washed off. The amount of labeled Ag is then measured. What assay is performed? - answer Competitive
Best describes competitive binding assays - answer A limited number of binding sites are present
identity the characteristics associated with non competitive enzyme immunoassays - answer Antibody is bound to the solid phase
Best describes homogenous assays - answer A washing step is not necessary
Testing situation where there is a direct relationship between the amount of bound label and the amount of patient antigen present - answer 1)Competitive radioimmunoassay using labeled antigen 2)Competitive enzyme immunoassay using labeled antigen
3)Noncompetitive enzyme immunoassay using a second labeled antibody
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice? - answer Wavelengths detected by microtiter plate reader
Which assay is the best choice for measuring small molecules, such as drugs and small
peptide hormones? - answer Enzyme multiplied immunoassay technique (EMIT)
Point of care testing is performed most easily using - answer Immunochromatographic assay
Compared with a capture assay, a radioimmunoassay is likely to be more advantageous
in terms of - answer Analytical sensitivity
The signal measured in a fluorescence polarization immunoassay is the - answer Orientation of emitted light waves
Rheumatoid factor can cause false positive results in which type of assay - answer Heterogeneous noncompetive
Extremely high analytical concentrations can cause false negatives results (hook effect) in which type of assay? - answer Heterogenous noncompetitive Statement about noncompetitive enzyme immunoassay is true - answer All patient antigen is allowed to react with binding sites
What compound is an appropriate label in chemiluminescent assay - answer Ruthenium derivative
In a capture assay to determine the presence of a viral antigen in a patient sample, which of the following would be on a solid phase - answer Unlabeled antibody
The use of vitamin of B7 in a delayed capture assay is found in which technique ? - answer Biotin avoiding labeling assay
An indirect ELISA assay was performed on a patients serum sample. What is the relationship of the amount of enzyme activity to the amount of patients antibody measured? - answer Directly proportional
An immunoassay is performed in the following way: Antigen is bound to a solid support. Patient serum is added, and patient antibodies bind to the antigen. The well is washed, and enzyme-labeled anti-immunoglobulin antibodies are added. The well is washed, and enzyme substrate is added. Enzyme activity is determined. Which immunoassay is described? - answer ELISA
When is the enzyme labeled antibody added in the sandwich immunoassay testing technique? - answer Right after incubation stage
The "sandwich" inthe sandwich immunoassay testing techniques is between: - answer 2 reagent antibodies
To detect low molecular weight analyzes, which immunoassay is best? - answer Homogenous immunoassay
A urine drug screen is performed using which technique below? (LO 9, T2) - answer Homogenous immunoassay
A out patient physicians office is investigating the addition of a convenient way to perform analysis for a commonly tested analyte where results are often needed before the physician enters the examination room. Which is most appropriate? - answer Rapid immunoassay
How is pregnancy testing performed at home? - answer Immunochromatography
------ is more sensitive?this assay is also sensitive enough to detect very low and very high analyte concentrations? - answer Heterogenous chemiluminescense
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