PSS 3421 Final Exam Questions & Answers 2024/2025
Cite evidence that DNA is carrier of heredity - ANSWERSHersey and Chase Radioactive viruses.
RESULTS: DNA carried hereditary info by transfer of viral DNA to bacteria causing formation of new viruses. Proteins on the other hand did not
stru...
Cite evidence that DNA is carrier of heredity - ANSWERSHersey and Chase Radioactive viruses.
RESULTS: DNA carried hereditary info by transfer of viral DNA to bacteria causing formation of new
viruses. Proteins on the other hand did not
structures of DNA - ANSWERSRibose, sugar, 4 nucleic acids
Key steps for replication - ANSWERSReplication began from a single origin of replication (Ori). DNA
strands are coiled around each other, and winding is needed. DNA replication occurs in both directions
from the origin of replication
Which of the following was true of experiments that demonstrated the DNA is the carrier of hereditary
information?
A. Adding viruses (phages) with radioactive DNA to E.coli resulted in bacteria that were radioactive,
because the viral DNA was transferred to the bacteria.
B. Adding viruses (phages) with radioactive proteins to E.coli resulted in bacteria that were radioactive,
because the viral protein was transferred to the bacteria - ANSWERSA
The building blocks of DNA are best called:
A. Deoxyadenosine, deoxycytidine, deoxyguanosine, deoxythymidine
B. asparagine, cysteine, glycine, tryptophan
C. Altrose, citrose, glucos, tagatose - ANSWERSA
Which of the following is avalid definition of a gene?
A. A sequence of amino acids that encodes for a complex protein.
B. A sequence of DNA that cods for a single polypeptide.
C. The smallest unit that gives rise to a trait - ANSWERSB and C
,Which of the following is true of E.coli DNA polymerase?
A. DNA polymerase III is the primary polymerase involved in DNA synthesis.
B. DNA polymerase III is a single polypeptide
C. DNA polymerase I is the primary polymerase involved in DNA synthesis.
D. DNA polymerase do not make mistakes, as this would have severe effects. - ANSWERSA
an enzyme capable of synthesizing DNA or RNA - ANSWERSpolymerase
an enzyme capable of degrading (removal of dNMP's) DNA or RNA, beginning at one end of the DNA or
RNA molecule - ANSWERSexonuclease
an enzyme capable of degrading DNA or RNA beginning at sites inside the DNA or RNA molecule -
ANSWERSendonuclease
Name 5 molecules that are requirements for DNA synthesis - ANSWERSDNA template
Primers
dNTPs (A,T,C,G)
DNA polymerase enzyme
Mg+2 cofactor for enzyme
DNA element that controls spatial temporal, and quantitative expression of one or more genes -
ANSWERSPromoter
A group of one or more prokaryotic genes transcribed into a single RNA molecule - ANSWERSoperon
cutting a eukaryotic gene transcript into a coding sequence, cutting out introns - ANSWERSRNA splicing
the site where RNA polymerase begins unwinding the two DNA strands - ANSWERSTATAAT
, sequence at -35 in a prokaryote where RNA polymerase first binds to the promoter - ANSWERSTTGACA
region that contains the sequence for a polypeptide chain - ANSWERScoding region
DNA to RNA to Protein - ANSWERSTranscription, translation
A student attempted to synthesize DNA in a test tube. She added the DNA that she wanted to copy, plus
the following ingredients: ATP, CTP, GTP, TTP, DNA polymerase III, and MG+2. The experiment Failed to
make more DNA. Why?
A. NTPS were used instead of dNTPs
B. DNA polymerase III was added instead of DNA polymerase I
C. A primer supplying a free 3' hydrzyl group was omitted - ANSWERSA and C
is a point mutation in which a single nucleotide change results in a codon that codes for a different
amino acid - ANSWERSMissense
a mutation in which a sense codon that corresponds to one of the twenty amino acids specified by the
genetic code is changed to a chain-terminating codon - ANSWERSNonsense
is a genetic mutation caused by indels (insertions or deletions) of a number of nucleotide in a DNA
sequence that is not divisible by three. - ANSWERSFrameshift
mutations in DNA that do not significantly alter the phenotype of the organism in which they occur -
ANSWERSSilent
Which of the following statements is the best one regarding translation?
A. translation occurs on ribosomes.
B. Translation requires mRNA transcript plus tRNAs
C. Translation is the copying DNA into RNA - ANSWERSA and B
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