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The Klenow fragment of E. coli DNA polymerase I is used: correct answersto label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? correct answersgel mobility shift assay _____ involves mixing a labeled oligon...

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ASCP MB: Extra-Practice Questions

The Klenow fragment of E. coli DNA polymerase I is used: correct answersto label DNA probes by random
primer labeling.



What is a solution hybridization method for the detection of nucleic acid:protein interactions? correct
answersgel mobility shift assay



_____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction
occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid
interaction. correct answersGel mobility shift assay



Which probe is most often used to detect trisomy 21 by interphase FISH? correct answersalpha satellite
DNA probe



_____ detect specific chromosomes and numbers of the specific chromosomes. correct answersAlpha
satellite DNA probe



Which reagent generates a signal during the annealing stage of a quantitative real time PCR reaction?
correct answersmolecular beacon



Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers.
Which sequencing technique is best suited to determine if methylation of cytosine residues has taken
place in the promoter region of a given gene? correct answersbisulfite sequencing



_____ is a modified chain terminator method specifically designed to detect methylated nucleotides.
correct answersBisulfite sequencing



Which method is best suited to screen for mutations in a gene having multiple possible mutations?
correct answerssingle strand conformational polymorphism (SSCP)

,_____ is a screening test in which the exact nature of a mutation does not need to be known in order to
detect the presence of a mutation. correct answersSingle strand conformational polymorphism (SSCP)



What is the best method to rule out the possibility of genomic DNA giving a false-positive result in an RT-
PCR reaction? correct answersuse of upstream and downstream primers that span an exon-intron-exon
region of the target



*amplicaons generated from genomic DNA would be larger than the specific amplicon generated from a
mature, spliced mRNA



A common use for pulsed-field gel electrophoresis is: correct answersepidemiological typing of bacterial
strains.



_____ after digestion of bacterial chromosomes with restriction enzymes is used to determine similarity
between bacterial isolates. correct answersPFGE



A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product associated with
CML?

a. 11;18

b. 14;18

c. 9;22

d. 9;14 correct answerst(9;22)



The presence of a distinct band along with the smear indicates at least 1% of the patient's B cells have a
_____ of the Ig heavy chain. correct answersmonoclonal rearrangement



A molecular-based test commonly used in testing donors and recipients prior to organ transplantation is:
correct answerssequence-specific PCR.



_____ is commonly performed to determine polymorphisms in class I and class II HLA-DRB and HLA-DRQ
genes. correct answersSequence-specific PCR

, DNA sequencing of hypervariable regions I and II is used to: correct answersdetect polymorphisms in
human mitochondrial DNA.



HV1 and HV2 are sequenced to determine _____. correct answersmitochondrial inheritance.



Repeated discrepancies between patient identifiers will result in _____ correct answersthe error being
documented to the laboratory supervisor and the risk manager will conduct a root cause analysis.



An index of precision is statistically known as the: correct answerscoefficient of variation.



The _____ is the measure of relative random error expressed as a percentage. correct answerscoefficient
of variation



The term used to describe reproducibility is: correct answersprecision.



_____ is the degree to which results of multiple analyses of the same specimen agree. correct
answersPrecision



The process by which an agency or organization uses predetermined standards to evaluate and recognize
a program of study in an institution is called: correct answersaccreditation.



Using a common labeling system for hazardous material identification such as HMIS or NFPA 704, which
hazard is represented by:



the top red quadrant?

the right yellow quadrant?

the lower white quadrant?

the left blue quadrant? correct answersflammability

reactivity

special reactivity

health

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