MIP302 - Final Exam 2024/2025 questions with answers
14 keer bekeken 0 keer verkocht
Vak
MIP
Instelling
MIP
MIP302 - Final Exam 2024/2025 questions with
answers
thermophiles - ANSWERS bacteria that live in environments with elevated temperatures (above 131°F)
super/hyper thermophile - ANSWERS bacteria that live in environments with extremely elevated
temperatures (above 300°F)
Differential Media...
microaerophilic bacteria - ANSWERS Require atmospheric oxygen but at reduced levels
facultative anaerobes - ANSWERS Grow with or without oxygen (better with oxygen)
obligate anaerobe - ANSWERS grow in absense of atmospheric oxygen
Ambient air - ANSWERS Normal air
candle jar - ANSWERS reduced levels of O2, higher levels of CO2
GasPak - ANSWERS reduced environment (devoid of O2)
,Redox indicators for Gaspak and Candle jar - ANSWERS Redox indicator methylene blue (blue when
oxidized, white in reduced state)
Catalase Test - ANSWERS To see if bacteria produces enzyme catalase; add 3% H2O2 to a DRY smear of
bacteria; produces bubbles=(+); delayed/no bubbles=(-)
Oxidase Test - ANSWERS To see if bacteria produces cytochrome oxidase (uses cytochrome C and
cytocrhome oxidase in ETC); uses Oxidrop as reagent; blue/maroon=oxidized, (+); colorless= reduced, (-);
3 MIN
PR Glucose Broth - ANSWERS Testing for glucose fermentation; Differential media (ability to ferment
carb); phenol red indicator (yellow in acidic, red in basic); contains peptone and beef extract in PR broth;
Durham tube put in tube to catch gas; yellow=(+); red=(-); 18-24 hours
Nitrate Broth - ANSWERS Differential media [ability to reduce nitrate (NO3) to nitrite (NO2)] using
anaerobic resp.; Nitrate A and Nitrate B reagents (react w/ nitrite--> red); RESULTS: red during first
round, (+) nitrate reductase, (-) nitrite reductase; Colorless during first round, inconclusive, add zinc; Red
during second round (Nitrate A, B, and zinc) (-) for nitrate and nitrite reductase; Colorless during second
round (+) nitrate and nitrite reductase; 48 HOURS
MacConkey Agar - ANSWERS To test for lactose fermentation of Gram (-) rods; differential media
(lactose fermentation); selective media (grows Gram - enteric bacilli, inhibits Gram - cocci and Gram +);
neutral red reagent (red in acidic, yellow in basic); contains peptone and beef extract; RESULTS:
red/dark-pink colonies surrounded by zone of bile salt, strong lactose fermenter; colorless colonies, do
not ferment lactose; if no growth, cannot make conclusions about lactose fermentation; 18-24 HOURS
Phenylethyl Alcohol (PEA) Agar - ANSWERS Test for Gram (+) growth; Selective (selective component
1/4 phenylethyl OH); not as stringent as MacConkey; RESULTS: growth, most likely Gram (+) but have to
do a Gram stain to check; 18-24 HOURS
Blood Agar (BAP) - ANSWERS Used to cultivate medically significant bacteria; all-purpose media;
differential (ability to hemolyze red blood cells); includes TSA and sheep red blood cells; RESULTS: BETA,
complete hemolysis, clear zone surrounding colonies; ALPHA, incomplete lysis, green-ish brown area
surrounding colony; GAMMA, no lysis; Don't look at colony itself to determine results; 24 HOURS
, Pseudosel Agar (Cetrimide Agar) - ANSWERS Used to isolate and identify Pseudomonas aeruginosa;
Selective (P. aeruginosa can grow, all others inhibited bc of cetrimide); Differential (produce pyocyanin);
can see under room lighting; RESULTS: blue/green colonies, positive result, P. aeruginosa; 18-24 HOURS
Pseudomonas Agar F - ANSWERS Used to differentiate bacteria based on ability to produce pigment
fluorescein (differential); all-purpose medium; contains magnesium and potassium salts (enhance
fluorescein production); should be visualized under UV, even tho can see green pigment in room
lighting; RESULTS: glowing under UV, produces fluorescein, (+); 18-24 HOURS
coliforms - ANSWERS Gram negetaive rods that ferment lactose and produce gas, facultative anaerobes,
and do not produce spores
tryptophanase - ANSWERS enzyme produced by bacteria that can use tyrptophan as a carbon source
tryptophan - ANSWERS catabolized into indole, pyruvic acid, and ammonia
tryptone broth - ANSWERS medium for indole test
48 - ANSWERS indole test is incubated for __ hours
Kovac's reagent - ANSWERS added to broth after incubation in indole test
present - ANSWERS if bacterium produces tryptophanase, indole will be ______ in the broth
absent - ANSWERS if bacterium does not produce tryptophanase, indole will be ______ in the broth
red ring - ANSWERS kovac's reagent reacts with the indole to produce a ___ ____ at the surface of the
broth (if positive)
Voordelen van het kopen van samenvattingen bij Stuvia op een rij:
Verzekerd van kwaliteit door reviews
Stuvia-klanten hebben meer dan 700.000 samenvattingen beoordeeld. Zo weet je zeker dat je de beste documenten koopt!
Snel en makkelijk kopen
Je betaalt supersnel en eenmalig met iDeal, creditcard of Stuvia-tegoed voor de samenvatting. Zonder lidmaatschap.
Focus op de essentie
Samenvattingen worden geschreven voor en door anderen. Daarom zijn de samenvattingen altijd betrouwbaar en actueel. Zo kom je snel tot de kern!
Veelgestelde vragen
Wat krijg ik als ik dit document koop?
Je krijgt een PDF, die direct beschikbaar is na je aankoop. Het gekochte document is altijd, overal en oneindig toegankelijk via je profiel.
Tevredenheidsgarantie: hoe werkt dat?
Onze tevredenheidsgarantie zorgt ervoor dat je altijd een studiedocument vindt dat goed bij je past. Je vult een formulier in en onze klantenservice regelt de rest.
Van wie koop ik deze samenvatting?
Stuvia is een marktplaats, je koop dit document dus niet van ons, maar van verkoper stuviagenius18. Stuvia faciliteert de betaling aan de verkoper.
Zit ik meteen vast aan een abonnement?
Nee, je koopt alleen deze samenvatting voor €13,93. Je zit daarna nergens aan vast.
