MIP 302 Exam 1 Questions with Revised Answers
What are some main mechanisms of chemotherapeutic agents? - Answer-1. inhibition of cell wall synthesis
2. inhibition of protein synthesis
3. inhibition of nucleic acid synthesis
4. cell membrane disruption
5. metabolic antagonism
In genera...
MIP 302 Exam 1 Questions
with Revised Answers
What are some main mechanisms of chemotherapeutic agents? - Answer-1. inhibition
of cell wall synthesis
2. inhibition of protein synthesis
3. inhibition of nucleic acid synthesis
4. cell membrane disruption
5. metabolic antagonism
In general, how do penicillin and other β-lactam antibiotics work? What cell types are
the most effective against? Why are they not harmful to humans? - Answer-they target
cell wall synthesis and we do not have cell walls
How does tetracycline work? Why are eukaryotic organisms less affected by
tetracycline compared to bacteria? - Answer-it binds with 30s subunit ribosome and we
have less 30s than prokaryotes
All of the aspects of the Kirby-Bauer test are standardized in such a manner that the
only variable being tested is the bacteria's interaction with the antibiotics. How did we
standardize this experiment? - Answer-1. incoulum
2. medium
3. antibiotic
4. incubation
5. interpretation
What is a McFarland standard? What does it tell you? How is it used? - Answer-is it
used to ensure that there is roughly the same about of bacteria in each sample
What is a zone of inhibition? - Answer-how much it kills
Why did you swab the entire plate several times (without dipping our swab back into the
inoculated saline)? Why was it important to have a confluent bacterial lawn? - Answer-
you want bacteria on the whole thing so we can see the zone of inhibitions
explain the difference between resistant and susceptible bacteria to a given antibiotic. -
Answer-resistant means that the bacteria did not die and susceptible means it did kill
the bacteria
, Why are some bacteria more resistant or more susceptible to certain antibiotics? -
Answer-depending on bacterial structure, what the antibiotic targets, and if they have
any mutations against the antibiotic
Why would you expect to see a much larger zone of inhibition for ampicillin for
Staphylococci than for a Gram negative bacteria? In other words, why would E. coli
have a smaller measurement of susceptibility compared to Staphylococci? - Answer-E.
coli has less ppg so it gets killed faster therefore needs a smaller zone of inhibition to be
considered susceptible
The five-kingdom classification system? - Answer-animalia
plantae
fungi
protista
monera
what falls into the animalia class? - Answer-animals
what falls into the plantae class? - Answer-plants
what falls into the fungi class? - Answer-molds
yeast
what falls into the protista - Answer-protozoa
algae
what falls into the monera class? - Answer-bacteria
what is a virus? - Answer-composed of only protein and nucleic acid (either RNA or
DNA but not both)
acellular
not part of the 5 kingdoms system
only replicate within living host cells
have no nutritional needs, are able to replicate and are infectious to other cells
what is a prion? - Answer-composed of only protein
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