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MB (ASCP) Exam Questions With Verified Answers. Predominant form of DNA - answerRight-handed B DNA and histone interaction is due to what type of bond? - answerIonic What stains to the minor groove of DNA duplex? - answerSYBR Green What is the most abundant type of RNA? - answerRibosomal RNA (...

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MB (ASCP) Exam Questions With Verified
Answers.


Predominant form of DNA - answer✔Right-handed B

DNA and histone interaction is due to what type of bond? - answer✔Ionic

What stains to the minor groove of DNA duplex? - answer✔SYBR Green

What is the most abundant type of RNA? - answer✔Ribosomal RNA (rRNA)

What does RNA act as in a retrovirus? - answer✔Genome and mRNA

What increases half-life of mRNA? - answer✔5' Methyl Cap

What kind of DNA is most likely to be transcriptionally active? - answer✔Euchromatin

What is the most common type of eukaryotic DNA? - answer✔Satellite DNA

What specimen has the most DNA according to molecular weight? - answer✔White Blood Cells

What amino acids compose histones? - answer✔Lysine and Arginine

Amino acid that all proteins start with: - answer✔Methionine

What protects the DNA strand during replication? - answer✔Single-stranded binding protein

Leading Strand - answer✔3' to 5' template

Lagging Strand - answer✔5' to 3' template

What DNA strand is being copied in transcription? - answer✔Antisense Strand

First step after newly formed mRNA is made? - answer✔Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
answer✔Telomerase

What polymerase has repair activity? - answer✔Beta/Epsilon

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What polymerase acts on leading strand? - answer✔Delta

What polymerase acts on lagging strand? - answer✔Alpha

Which polymerase is used for mitochondrial replication and repair? - answer✔Gamma

What polymerase binds the 5' end to 3' end of dsDNA? - answer✔Topoisomerase

Which RNA polymerase is mainly for transcription? - answer✔RNA Pol II

Which polymerase is involved with mRNA synthesis? - answer✔RNA Pol II

What inactivates RNAses? - answer✔Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6
hours at 400C

What type of restriction enzyme recognizes palindromic sites? - answer✔Restriction Enzyme
Type II

What type of restriction enzyme lacks methylation activity? - answer✔Restriction Enzyme Type
II

What is necessary to make cDNA? - answer✔Reverse transcriptase

Number of SNPs in a human genome? - answer✔11 billion (10^7)

How many genes (base pairs) are in a human haploid genome? - answer✔3 billion base pairs

The polymerization of acrylamide requires: - answer✔Catalyst (APS or TEMED) or Light
activation

How does agarose gel pore size relate to concentration? - answer✔Inversely proportional

What is denatured during a southern blot? - answer✔Hydrogen bonds (inter-strand bonds)

Melting temperature formula - answer✔Tm = (4C x GC Pairs) + (2C x AT pairs)

Which is likely to interfere with fluorescent probe? - answer✔5' G

Proper GC content of primer - answer✔35-65%

Inhibitors of PCR - answer✔Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol
Blue

Order to add to a PCR reaction: - answer✔Master mix > patient sample > positive control >
negative control

Pre Area - answer✔Positive Pressure

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Post Area - answer✔Negative Pressure

Which PCR requires two steps for specificity? - answer✔Nested PCR

Which microorganism is detected by branched DNA amplification? - answer✔DNA or RNA

Which technique uses RNA, antibodies, and DNA? - answer✔Hybrid Capture Assay

Use of bisulfite treatment of DNA to determine its pattern of methylation - answer✔Bisulfite
DNA

What tests are isothermal? - answer✔NASBA, TMA, & SDA

Which leukemia is associated with FLT3 gene? - answer✔AML

T-cell gene rearrangement is seen in what malignancy? - answer✔Sezary Syndrome

Which translocation causes Acute Premyelocytic Leukemia? - answer✔t 15;17

Ewing Sarcoma - answer✔t 11;22

Synovial Sarcoma - answer✔t X;18

Follicular Lymphoma - answer✔t 14;18

What genes would be screened in a colorectal panel? - answer✔MSH2 & MLH1

What gene mutation causes a high risk of breast or ovarian cancer? - answer✔BRCA1 and
BRCA2

What does MSI test for? - answer✔Mutations in mismatch repair genes (MMR)

What disease is related to factor V coagulation gene (F5 1q23)? - answer✔Thrombossis

How many copies are in a full Fragile X mutation? - answer✔200-2000+ CGG repeats

What disease is known for numerous mutations and large deletions? - answer✔Muscular
Dystrophy (Duchenne)

What HPV causes cervical cancer? - answer✔16 & 18
You receive a paraffin embedded specimen for HPV typing, which method should you use? -
answer✔PCR

Significant change in HIV viral load: - answer✔0.5 log10
Lowest viral concentration that can be detected over background or a negative control in the test
assay - answer✔Limit of Detection (LOD)

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