Laboratory Animal Science Exam Questions with Verified Answers
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Laboratory Animal Technician
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Laboratory Animal Technician
What is Article 1 and what function does it have? - Answer-Experiments on animals act, to protect the animals
What do article 1A and 1D stand for? - Answer-1A: all animals have an intrinsic value
1D: animal experiments are forbidden, unless 3Rs are considered
What is the definition of an ani...
laboratory animal science exam questions with veri
what is article 1 and what function does it have
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Laboratory Animal Science Exam
Questions with Verified Answers
What is Article 1 and what function does it have? - Answer-Experiments on animals act,
to protect the animals
What do article 1A and 1D stand for? - Answer-1A: all animals have an intrinsic value
1D: animal experiments are forbidden, unless 3Rs are considered
What is the definition of an animal experiment? - Answer-Any invasive or non-invasive
use of an animal for experimental or other scientific purpose with known or unknown
outcome, or educational purposes, which may cause the animal a level of pain,
suffering, distress or lasting harm equivalent to or higher than the introduction of a
needle according to good veterinary practice
When is an animal experiment not allowed? - Answer-If the 3Rs are not met
How does the legislation in the EU deviate from Dutch legislation? - Answer-In the EU
animals which are killed for the use of organs or tissues are excluded
What are the three national involved parties of control? - Answer-CA (CCD): give ethical
judgement and approve the project licence
NVWA: give the institutional licence, provide external supervision and yearly registration
NCad: advice the minister, CCD and AWBs
What are the three component of a project licence application? - Answer-A non
technical summary
A project proposal
Appendices with details about the animal experiments
What is the function of the animal welfare body? - Answer-Advice and supervision of
animal experiments
Who grants the project licence? - Answer-Centrale commissie dierproeven (CCD)
What is in the working protocol? - Answer-Details of experiments
Method of housing
Level of discomfort
Humane end points
Method of killing
Agreements/responsibilities
Amendments
What are the four levels of discomfort - Answer-mild, moderate, severe, non-recovery
, name 3 reasons to stop an animal experiment - Answer-1. The result has been
achieved
2. The animal suffers severly and unintentionally
3. The animal is no longer suitable as a model or results can no longer be achieved
Name the general parameters of severe and unintentional suffering - Answer-Deviant
behaviour
Body Weight
Locomotion, mobility
Food and water intake
General body condition
Why is having clear indicators of animal suffering important for the researcher? -
Answer-Animals in bad condition can give unreliable results or increase the variation
Spontaneous death prevents the possibility to acquire data from the animal
Who provides external supervision over animal welfare? - Answer-NVWA, regular
inspection visits
Who provides internal supervision over animal welfare? - Answer-Care taker/technician,
AWB, Veterinarian
Give a few examples of replacement of animal tests - Answer-In vitro techniques
(organelles, cell lines)
What are some of the advantages of using an in vitro system over an animal testing -
Answer-There is no interaction with other organs, better to control the conditions, more
flexible, small quantity of test substance needed
What are some disadvantages of using an in vitro system over an animal test - Answer-
Interactions with other organs are unknown
Difficult to extrapolate
What is the definition of a reduction - Answer-a means of lowering the number of
animals used
What is the definition of a refinement - Answer-the method of lowering the disomfort of
animals by modifying animal husbandry or experimental procedures
What three disease models exist - Answer-Induced (experimental) disease models -
induce obesity or tumor growth
Spontaneous (genetic) disease models - nude mice/hypertensive rat
Transgenic disease models - knock out/knock in
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