100% tevredenheidsgarantie Direct beschikbaar na betaling Zowel online als in PDF Je zit nergens aan vast
logo-home
Summary Introduction and microbial growth €2,99
In winkelwagen

Samenvatting

Summary Introduction and microbial growth

 26 keer bekeken  0 keer verkocht

Summary about the lecture and corresponding literature about introductionary microbiology and microbial growth.

Voorbeeld 2 van de 8  pagina's

  • Nee
  • 5
  • 18 januari 2021
  • 8
  • 2020/2021
  • Samenvatting
book image

Titel boek:

Auteur(s):

  • Uitgave:
  • ISBN:
  • Druk:
Alle documenten voor dit vak (15)
avatar-seller
stijnvantrijp
HC3 Introduction and Microbial
growth (BOOK)
Chapter 5.1 – 5.7

CH5 Microbial Growth and Regulation
5.1 Binary Fission, Budding, and Biofilms
In microbiology, growth is defined as an increase in the number of cells.
Binary fission means that an cell elongates to about twice its original length and then a septum (kind
of ‘insnoering’) forms and finally completes and the cell divides.

The time required for a cell to divide and thus form a new generation, is called the generation time.

Most bacteria divide by binary fission, but there are other forms of cell division. Budding divisions
form a totally new daughter cell, with the mother cell retaining its original identity. The big difference
between budding bacteria and bacteria that divide by binary fission is that the formation of new cell
wall material in budding division occur at one point (polar growth) while in binary fission it happens
in the whole cell (intercalary growth). Note that for polar growth, large internal membrane
complexes are not partitioned and also need to be formed de novo in the developing bud.

Biofilms
Microbial cells can grow either in suspension or attached to surfaces. The suspended live style (swim
or float in liquid medium) is called planktonic growth. Microorganisms that grow attached to a
surface (sessile growth) can develop into biofilms which are attached polysaccharide matrixes
containing embedded bacterial cells. Biofilms form in stages:
1. Attachment of planktonic cells
2. Production of sticky matrix
3. Further growth and development to form the tenacious and nearly impenetrable mature
biofilm

Some biofilms form multiple layers with different organisms inside the individual layers these
biofilms are called microbial mats.

5.2 Quantitative aspects of microbial growth
In the generation time, both total cell number and cell mass double.
When the cell number of exponential growth is plotted on a logarithmic scale as a function of time
(semilogarithmic graph) the points fall on a straight line. This reflects the fact that the cells are
growing exponentially and that the population is doubling in a constant time interval.

The relationship between the initial number of cells in a culture and the number present after a
period of exponential growth: N = No x 2n
Where N is the final number, N0 the initial number and n the number of generations.
Generation time: g = t / N

The instantaneous growth rate constant (k) expresses the rate at which the population is growing at
any instant, expressed in units of reciprocal hours (h -1). dN/dt = kN by integrating natural logarithms;
N = No x ekt

, 5.3 The microbial growth cycle
A culture of organisms growing in an enclosed vessel, is called a batch culture.

Growth when a microbial culture is inoculated into a fresh growth media only begins after a period of
time, this time is called the lag phase. When a growing culture is tranferred into the same medium
under the same conditions, there will be no lag phase.

In general, prokaryotic cells grow faster than eukaryotes, and small eukaryotes tend to grow faster
than large ones.

Stationary and death phases
When exponential growth ceases (due to essential nutrient depletion or waste products
accumulation), the population enters stationary phase (there is no increase or decrease in cell
number —> growth rate = 0).
Cryptic growth means that some individuals divide, while others die —> net decrease/increase = 0.
During the death phase, the growth number < 0.




5.4 Continuous culture
The environment in batch cultures are constantly changing because of nutrient consumption and
waste production. These limitations can be circumvented in a continuous culture device.

The most common type of continuous culture is the chemostat, a device in which growth rate and
cell density can be controlled independently.
Two factors govern the specific growth rate (the dilution rate (D) = F / V where F is the flow rate and
V culture volume) and cell density (concentration of a limiting nutrient present in the sterile medium
entering the chemostat vessel).

In steady state, the specific growth rate equals D; the rate of increase in cell numbers due to growth
is equal to the rate of decrease in cell numbers due dilution (outflow).

Voordelen van het kopen van samenvattingen bij Stuvia op een rij:

Verzekerd van kwaliteit door reviews

Verzekerd van kwaliteit door reviews

Stuvia-klanten hebben meer dan 700.000 samenvattingen beoordeeld. Zo weet je zeker dat je de beste documenten koopt!

Snel en makkelijk kopen

Snel en makkelijk kopen

Je betaalt supersnel en eenmalig met iDeal, creditcard of Stuvia-tegoed voor de samenvatting. Zonder lidmaatschap.

Focus op de essentie

Focus op de essentie

Samenvattingen worden geschreven voor en door anderen. Daarom zijn de samenvattingen altijd betrouwbaar en actueel. Zo kom je snel tot de kern!

Veelgestelde vragen

Wat krijg ik als ik dit document koop?

Je krijgt een PDF, die direct beschikbaar is na je aankoop. Het gekochte document is altijd, overal en oneindig toegankelijk via je profiel.

Tevredenheidsgarantie: hoe werkt dat?

Onze tevredenheidsgarantie zorgt ervoor dat je altijd een studiedocument vindt dat goed bij je past. Je vult een formulier in en onze klantenservice regelt de rest.

Van wie koop ik deze samenvatting?

Stuvia is een marktplaats, je koop dit document dus niet van ons, maar van verkoper stijnvantrijp. Stuvia faciliteert de betaling aan de verkoper.

Zit ik meteen vast aan een abonnement?

Nee, je koopt alleen deze samenvatting voor €2,99. Je zit daarna nergens aan vast.

Is Stuvia te vertrouwen?

4,6 sterren op Google & Trustpilot (+1000 reviews)

Afgelopen 30 dagen zijn er 52510 samenvattingen verkocht

Opgericht in 2010, al 14 jaar dé plek om samenvattingen te kopen

Start met verkopen
€2,99
  • (0)
In winkelwagen
Toegevoegd