Maldi tof - Samenvattingen, Aantekeningen en Examens

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CMMB 461 FINALBasic components of mass spec - correct answer 1. Ion source 2. mass analyzer 3. detector Two main types of ionization - correct answer 1. Electrospray ionization 2. MALDI Electrospray Ionization (ES) - correct answer Peptide solution is passed through a narrow metal needle with a high electrical potential resulting in highly charged droplets of protonated peptide ions. Matrix-assisted laser desorption ionization (MALDI) - correct answer Sample is co-crystallized wi...

Pasteurella correct answers 1) 17 species 2) Widespread in wild and domesticated animals 3) *Non-motile* Gram-negative coccobacilli/bacilli 4) Facultative anaerobes 5) Oral flora of animals What are the three subspecies of P. Multocida? correct answers P. Multocida, P. Septica, and P. Gallicida P. Multocida Habitat correct answers 1) Normal oral microbiota of cats, dogs, and birds 2) Zoonosis 3) P. multocida is the most frequently isolated species from human specimens. 4) Infection ...

CMI correct answers immune system response that is important for fighting off intracellular parasites (FIPs and OIPs), because once they are inside of the cells antibodies are not helpful mycobacterium characteristics correct answers -have a lot of hydrophobic myconic fatty acids in their cell wall, so they do not gram stain well -gram positive like bacilli -obligate aerobes -slow growers -colonies take 4-6 weeks to appear -produce dry, flat colonies with irregular filamentous edge that ...

Instrumental Analysis ACS Exam 2023 How to calculate signal to noise S/N relationships Beer's Law Beer's Law Calculations Hard ionization vs soft ionization MS: hard ionization sources MS: hard ionization sources examples Electron Impact (EI) EI advantages EI disadvantages MS: soft ionization sources MS: soft ionization sources examples Chemical ionization (CI) fragmentation in CI CI advantages CI disadvantages Electrospray ionization (ESI) MALDI abreviation MALDI Theory MS...

The advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture - can be automated for high volume processing MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests -pathogen-same method for all bacteria and fungi -cheaper cost per sample Describe the precautions that the State lab takes to prevent contamination (3) - Answer -designated clean (pre) and dirty (pos...

Learning Objectives • Explain the translation process from mRNA to amino acid • Explain post-translational modification of proteins • Explain protein synthesis processing in the ribosome • Explain the primary, secondary, tertiary and quaternary structures of protein • Explain the basic principles of mass spectrometry (MALDI-TOF) Introduction In the Protein Synthesis lab, you will learn about the difference between protein synthesis in prokaryote (using E. coli) and eukaryote (us...

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MBB331 MIDTERM 1 - Spring 2017 ANSWER KEY NAME (PRINT in block letters):____________________________________________ SFU ID: ________________________________________________________________ TA/TUTORIAL DAY:TIME _______________________________________________ Answer all questions in the spaces provided. Keep your answers concise and legible. If your writing is not legible, you will not be given any marks for your answers. Exam total = 45 marks Section 1. Multiple choice and True/False ques...

(MB) ASCP Study Exam Questions Answered 100% correct What is metagenome? Sampling the genome sequence of a community of organisms. Polymorphism involved for Irinotecan? UGT1A1 NGS related to deletions and insertions, coverage, depth? -Paired-end reads for detection of indels -Coverage is how much of your target has been read -Depth is how many times a unique position has been covered Polymerase for lagging strand? Euk - DNA Pol Alpha Pro - DNA Pol I Polymerase...