Molecular cloning - Samenvattingen, Aantekeningen en Examens

BIT 510 MidtermWhich of the following best describes the traditional central dogma of molecular biology? A. DNA is transcribed into mRNA which is translated into protein B. DNA can be produced by replication or reverse transcription C. DNA is replicated into RNA, some of which is translated into protein D. DNA is replicated into mRNA which is transcribed into protein - correct answer A. DNA is transcribed into mRNA which is translated into protein What is the end goal of cloning the Ca...

A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features except: A) a selectable antibiotic marker such as kanamycin resistance. B) a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice. C) a Ti plasmid lacking its T-DNA segment. D) active vir gene products from the altered Ti plasmid. E) an ability to induce crown gall formation in infected leaves. - Answer-B) a shuttle vector with 25 bp TDNA repeats ...

BISC 440 Lab 5: Molecular Cloning questions and answers 100% accurate The RAD52 gene can be isolated from the yeast cells. What should we do first so we can isolate the gene we need? a. separate the DNA using gel electrophoresis b. perform DNA sequencing c. use a restriction enzyme d. perform DNA extraction d In order to isolate RAD52, what should we do after extracting all DNA from the yeast cells? a. sequence the DNA b. proceed to the ligation step c. transform the yeast cells ...

UAMS Molecular Diagnostics Exam 1 Questions & Answers 2024/2025 Which of the following is NOT an application of molecular diagnostic testing? Cloning humans Identifying bacterial pathogens Detecting genetic disorders Paternity Testing - ANSWERSCloning humans Who is considered the "father of modern genetics"? Erwin Chargaff Frederick Griffith Friedrich Miescher Gregor Mendel - ANSWERSGregor Mendel He isolated nuclein. Frederick Griffith Friedrich Miescher Gregor Mend...

recombinant DNA correct answers DNA from 2 sources is known as procedure that leads to many copies correct answers gene cloning vector correct answers a carrier for DNA that is to be cloned plasmid, viral correct answers What are the 2 most common vectors? DNA ligase correct answers _______________________ must be used to permanently link the DNA before it is introduced into a host cell. restriction enzymes correct answers When performing gene cloning, the vector and the DNA of in...

Application of Biotechnology for Plant Breeding & Genetics 2 o Gene cloning o Plant transformation o Molecular analysis o Genomic tools o Marker assisted breeding o Course orientation o Why alter plants o Why use biotechnology o Biotechnology strategies Outline 3 o Course orientation o Why alter plants o Why use biotechnology o Biotechnology strategies Outline 5 Dr. Guo-qing Song Associate Professor, Associate Director of PBROC Department of Horticulture A336, PSS Bldg P...

BIOL 1040 Unit 4 Week 7 Molecular Cloning Lab

Describe DNA structure - Answer-DNA forms a double stranded helix and adenine pairs with thymine, and cytosine pairs with guanine What pair forms hydrogen bonds and how many - Answer-thymine/adenine form 2 bonds guanine/cytosine form 3 bonds Explain "the central dogma" - Answer-DNA turning into RNA turning into Protein What is transcription - Answer-Copying of DNA code to RNA, one nucleotide at a time so it is complementary to the DNA What is translation - Answer-RNA gets translated int...

A compound containing N-acetylneuraminic acid (sialic acid) is: A) cardiolipin. B) ganglioside GM2. C) phosphatidylcholine. D) platelet-activating factor. E) sphingomyelin. - Answer-b A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n): A) E. coli chromosome. B) messenger RNA. C) plasmid. D) yeast "ARS" sequence. E) yeast transposable element. - Answer-c A hydropathy plot is used to: A) determine the water-solubility of a protein. B) deduce the qu...

Recombination CORRECT ANSWER the process of forming recombinant DNA Recombinant DNA (rDNA) CORRECT ANSWER a new sequence of DNA made by combining two pieces of DNA Crossing over CORRECT ANSWER sections of homologous chromosomes are swapped to form rDNA in preparation for gamete production Recombinant DNA technology CORRECT ANSWER a method of genetic engineering where pieces of DNA are combined to form rDNA in a lab Restriction enzymes CORRECT ANSWER enzymes that cut double-stranded D...