Pcr product - Samenvattingen, Aantekeningen en Examens

WGU Biochemistry OA (Latest 2022/2023) Verified Answers Bohr Effect chart (low pH) C- High CO2 H- High H+ A- Acidic R-Release of O2(rt. Shift) T- tense state (in tissues) Primary structure Sequence of amino acids that make up a protein Secondary Structure Sequence of amino acids fold. Tertiary Structure -more folding in a 3D shape. -R groups (side chains) Quaternary Structure Protein has more than 1 polypeptide chain. Chemical reaction -absorb and release en...

ASCP PRACTICE EXAM 2024 WITH 200 ACTUAL EXAM QUESTIONS AND DETAILED 100% CORRECT ANSWERS | SCORED A+ PCR that is not a limitation of real-time PCR? Select one: a. Requirement of a large initial sample b. Inability to quantitate DNA product due to endpoint analysis only c. Ability to detect PCR product during the plateau stage - ANSWER- B. Inability to quantitate DNA product due to endpoint analysis only A patient has the following test results:ANA = positive 1:320RA = positiveComplemen...

Lab 1 - Exercise 1: Primer Analysis and PCR Trouble-Shooting - Answer Compared and contrasted two primer pairs to find the qualities that contribute to the likelihood of a given primer pair producing the desired product. - NetPrimer Lab 1 - Exercise 2: Primer Design from a Target Sequence - Answer 1. Nucleotide BLAST (BlastN): identify the sequence. --> indicate organism of origin, size of assigned DNA sequence, and the name of the protein it encodes.

What is the most important factor in a successful PCR reaction? - Well designed primers What can the theory of primer design also be applied to? - The use of oligonucleotides in other techniques that utilize annealing or formations of duplexes of nucleotides (probes in Southern blot, Northern Blot, ect.) What variables influence the success of a PCR reaction? - - quality of template DNA - cycling conditions (melting temp., duration) - specificity and stability of the PCR primers What ...

BIOC LAT 5 QUESTIONS AND ANSWERS ALREADY PASSED A glucose molecule that has been fully metabolised (gone though glycolysis, citric acid cycle and electron transport chain) gives rise to a total of how many ATP molecules? 32 A high carbohydrate snack is consumed following a period of fasting. Which hormone would be regulating carbohydrate metabolism following the snack? insulin A restriction enzyme is an: endonuclease that cuts both DNA strands at a unique sequence A restriction ...

American Heart Association protocols are based on __________. Select one: A. theories ready for field trial B. trends in the EMS community C. opinions from a core group of cardiologists D. evidence-based research - ANSWER D. evidence-based research As an EMT, you may be authorized to administer aspirin to a patient with chest pain based on: Select one: A. medical director approval. B. the patient's condition. C. an order from a paramedic. D. the transport time to the hospital. - AN...

Which level of protein structure is disrupted through the hydrolysis of peptide bonds? Quaternary Tertiary Primary Secondary - ANSWER Primary The primary structure of a protein is the sequence of amino acids held together by peptide bonds. Peptide bonds are formed by dehydration reactions and disrupted by hydrolysis. A mutation in the beta-hemoglobin gene, which results in the replacement of the amino acid glutamate in position 6 with the amino acid valine, leads to the develop...

Among its many other uses, PCR is finding important applications in early clinical diagnosis of disease. An example is in testing for the presence of viruses to which antibodies do not appear until a long time after infection. Describe how PCR could be used to detect HIV infection at an early stage, including what information about HIV would be needed to begin with. PCR could be used to detected HIV by performing PCR on cells with primers that are designed to amplify the viral HIV DNA that...

MLT 106 Unit 2 Questions with All Correct Answers What are the DNA repair systems? - Answer-Photoreactivation, excision repair, recombinational repair, mismatch repair, SOS repair When can thymine bases be altered and what does it develop? - Answer-After exposure to UV radiation to develop thymine dimers Photoreactivation repair - Answer-Involves a photoreactivation enzyme (PRE) that cleaves the bonds between thymine dimers Supplies correct strand of DNA when an error is deleted - A...

Biochemistry WGU C785 FINAL EXAM Questions With Correct Answers Latest 2024 GRADED (Biochemistry Units 2-7) What is the basic structure of an amino acid? What do they look like? - Answer amino group (NH2 or NH3), carboxyl group (COO or COOH), alpha carbon (C), and variable group How do you identify the 3 different types of side chains: non-polar/hydrophobic, polar, and charged? - Answer Non-polar/hydrophobic - end with CH or "can't have" water. Polar - end with OH, SH, or NH. Charged - e...