Fluorescent protein - Samenvattingen, Aantekeningen en Examens

CMM 577 EXAM 2 2024 WITH 100% CORRECT ANSWERSWhat are the three different cytoskeletal elements? Describe them. - correct answer Actin: Responsible for cell protrusions Concentrated at the cell cortex Organize into bundles/networks Microtubules: Long, larger, Made of tubulin Give rise to cilia, mitotic spindles and interphase array Intermediate Filaments: Ropelike fibers made of IF proteins Mechanical strength and support for the entire cell and nuclear lamina Describe the classifi...

FISH - Cells fixed in place Fluorescently labeled probes consisting of 165 rRNA gene sequence sent to attach to RNA View in Epifluorescence microscope Cultivation independent methods - DNA from unculturable bacteria can be amplified and sequenced by PCR Sequences can be used to produce fluorescent probes that will bind to complementary DNA (Fluorescent In Situ Hybridization FISH) Microbial consortia - Microbes that can't grow on their own Metagenomics - DNA is isolated from an envir...

Last Universal Common Ancestor (LUCA) CORRECT ANSWER Was a microbe of unknown type that lived about 4 billion years ago; the origin of all organisms. What is 16s small unit rRNA used for? CORRECT ANSWER It's used to study construct phylogenetic trees of organisms because it's highly conserved and hasn't changed much over time. Antonie von Leeuwenhoek CORRECT ANSWER Father of microscopy in 1620 Increased surface area like folded membranes CORRECT ANSWER How do larger cells maximize di...

ASCP MLT (medical laboratory technicians) Exam With Complete Solutions Which tumor marker is used for colorectal cancer? [Accurate Ans: - CEA What is the half-life of IgG? Why is the duration of the half-life significant? [Accurate Ans: - 18-23 days; it binds with neonatal Fc receptor Which of the following is the most suitable specimen for the isolation of Bordetella pertussis? [Accurate Ans: - Nasopharyngeal swab Potassium, calcium, and total protein are ___________ in capillary spec...

BIOL 266 Final Exam 2024 Actual Questions and Answers Graded A   BIOL 266 Final Exam 2024 Actual Questions and Answers Graded A = Correct answer = We don’t have the answer Exam 1: MULTIPLE CHOICES: 1. You have isolated a mutant line of mammalian cells deficient in emerin. This mutant line is expected to be impaired in: A. Export of 40S and 60S ribosomal subunits from the nucleus B. Ribosome assembly in the nucleolus C. Transcription of some genes in certain ti...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Light Microscopy CORRECT ANSWER Used alongside differential staining i. Gram Staining: identifies gram (+) or gram (-) ii. Fluorescent stains: used to study molecule interaction/aggregation in certain tissue, cell, etc. 1. GFP used to study protein localization Cryo-electron microscopy CORRECT ANSWER Used to visualize objects (e.g. individual atoms) and finer structural cellular details much smaller than those resolved optically Cryo-electron tomography CORRECT ANSWER Creates 3D visua...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

ASCP MLS FINAL Exam Solved 100% Correct /Verified Answers 2024 Sodium determination by indirect ion selective electrode is falsely decreased by: A. elevated chloride levels B. elevated lipid levels C. decreased protein levels D. decreased albumin levels {Ans: - B. elevated lipid levels The chemical composition of HDL-cholesterol corresponds to: {Ans: - Triglyceride: 5%; Cholesterol: 15%; Protein: 50% In the Jaffe reaction, creatinine reacts with: A. alkaline sulfasalazine...

Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it means to say something is alive. Which of the following can be described as the smallest living unit? a) organelle b) DNA c) Cell d) protein - Answer-c The central dogma provides a framework for thinking about how genetic information is copied and ...