Western blotting - Samenvattingen, Aantekeningen en Examens

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Native-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, thus, you can estimate the protein's molecular ma...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. D. They can be produced in either liv...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...

An experiment with bacteria that has been exposed to an antibiotic reveals that the translocation step has been inhibited. Which antibiotic was likely used? correct answers none of these answers are correct. The correct mRNA secondary structure of transcriptional termination is __________ stem-loop structure. correct answers 3-4 In negative autoregulation, correct answers proteins repress their own gene expression. Which sequence is MOST likely to be prenylated in the ER? (The ... repre...

©THEBRIGHT EXAM STUDY SOLUTIONS 8/21/2024 8:36 PM MCB3020 Exam 3 Questions With Correct Answers Reverse transcriptase process - answer1. poly-T primer to single-strand RNA 2. reverse transcriptase to produce DNA-RNA hybrid 3. enzyme RNaseH to cut RNA and regenerate RNA primers 4, DNA polymerase and ligase synthesizes new complementary DNA strand cloning vector - answerallows for transfer of gene of interest to a host organism; allows for the making of recombinant DNA good cloning vecto...

Buffer Order and Henderson Hasselbach - Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - 3.6 Super secondary structure - helix turn helix parallel vs. antiparallel - parallel wedges face same way antiparallel face opposit...

what is the purpose of Polymerase chain reaction (PCR)? correct answer: diagnostic tool to amplify a desired DNA fragment. What do you need for PCR? correct answer: Excess PRIMERS, Excess dNTPs, DNA polymerase (taq) What is the purpose of DNA polymerase ? correct answer: makes polymers of any molecules what is a chain reaction ? correct answer: a reaction that progress in an exponential way 2--4--6--8--10 why making copied of DNA ? correct answer: diagnosing infections, crime ...

Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease Tyede H. Schmidt-Schultz1 and Michael Schultz2, * 1Department of Biochemistry, University of Go¨ ttingen, D-37075 Go¨ ttingen, Germany 2Department of Anatomy, University of Go¨ ttingen, D-37075 Go¨ ttingen, Germany * Corresponding author e-mail: Abstract For the first time we have extracted, solubilized and identif...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...