Western blotting - Samenvattingen, Aantekeningen en Examens

AAMC full length 2, EXAM Overviews 2023 Update amide linking is peptide on the carboxyl side - ANS-so amide linkage involving the N atom in AMC used to covalently attach flurophore to the peptide what expression gives the amount of light energy (J/photon) that is converted to other forms between the fluorescence excitation and emission events? - ANS-E=hf = hc/lambda, here we see that excitation is at 360 nm but observation is at 440 nm. so you have E = (6.62 x 10^-34)(3x 10^8)x (1/360...

Define biotechnology - Answer- The use of technology to improve living organism and processes for the bennifit of man Briefly state the main difference of modern from classical biotechnology - Answer- Modern BTEC involves recombinant DNA technology Selective breeding is an early form of classical biotech, where there is deliberate crossing of parent organisms with favorable traits. Name two vegetables that are products of selective breeding from one common ancestor that were shown in the l...

ACS Biochemistry First Semester Exam Questions with All Correct Answers Buffer Order and Henderson Hasselbach - ANSWER Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - ANSWER Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - ANSWER 3.6 Supe...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* Difficulty: Easy ASM Standard: N/A 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. ...

MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DN...

Biochemistry ACS Review Questions With 100% Complete Solutions A codon is present in _______. An anticodon is found in _______. The 5' - AUC - 3' codon, will base pair with the 5' - _______ - 3' anticodon. A. mRNA, tRNA, UAG B. mRNA, tRNA, GAT C. DNA, mRNA, GAU D. mRNA, tRNA, GAU E. DNA, mRNA, CUA - Answer-D. mRNA, tRNA, GAU Anticodon is reversed because it is running 5'-3 /.A researcher wants to determine if cancer cells express protein X. Which technique would be the mos...

What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel the size of the DNA fragment What factors affec...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. D. They can be produced in either liv...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

Laboratories that work with moderate risk pathogens are classified as BSL-2 Bacteremia is a term used to indicate the presence of bacteria in the blood. 00:29 01:22 Which of the following must be accomplished FIRST in order to conduct RT-PCR? Complementary DNA must be synthesized from RNA The most common of all nosocomial infections are ________ infections urinary tract MacConkey agar and eosin-methylene blue (EMB) agar are ________ media. both selectiv...