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Lab 6: Electrophoresis gels Questions Answers
DNA strands - ANSWERSdouble stranded 
 
gel electrophoresis - ANSWERSProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel 
 
uses: 
sort DNA strands according to length 
separating other types of molecules like proteins 
 
"gel" - ANSWERSis the filter that sorts the DNA strands; 
 
like a sponge made of jell-o w/many small holes in it 
 
electrophoresis - ANSWERSA process where DNA f...
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DNA strands - ANSWERSdouble stranded 
 
gel electrophoresis - ANSWERSProcedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel 
 
uses: 
sort DNA strands according to length 
separating other types of molecules like proteins 
 
"gel" - ANSWERSis the filter that sorts the DNA strands; 
 
like a sponge made of jell-o w/many small holes in it 
 
electrophoresis - ANSWERSA process where DNA f...
Gel Electrophoresis Virtual Lab Questions & Answers Correct!!
Why can't you physically sort the DNA by size? - ANSWERSDNA is too small 
 
What is the ultimate goal of Gel Electrophoresis? - ANSWERSto measure and sort DNA strands according to length 
 
What is the job of the Gel? - ANSWERSIt's the filter that sorts the DNA strands 
 
Explain how the electrical current separates the DNA. - ANSWERSThe electrical current makes the DNA move 
 
Explain how the strands of varying sizes "sort themselves". - ANSWERSThe short strands move through the holes quick...
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Why can't you physically sort the DNA by size? - ANSWERSDNA is too small 
 
What is the ultimate goal of Gel Electrophoresis? - ANSWERSto measure and sort DNA strands according to length 
 
What is the job of the Gel? - ANSWERSIt's the filter that sorts the DNA strands 
 
Explain how the electrical current separates the DNA. - ANSWERSThe electrical current makes the DNA move 
 
Explain how the strands of varying sizes "sort themselves". - ANSWERSThe short strands move through the holes quick...
DNA Biology & Technology: Gel Electrophoresis Questions & Answers
What is the purpose of installing the comb? - ANSWERSTo create a well or pockets in the gel where samples can later be placed. 
 
Why is ethidium bromide added at this step? - ANSWERSEthidium Bromide is needed to see the DNA bands in the gel under UV illumination. 
 
Why is it important to pour slowly and avoid air bubbles? - ANSWERSSuch bubbles would interfere with the movement of the sample through the gel, distorting the results. 
 
What could happen if you do not get a new tip to load each s...
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What is the purpose of installing the comb? - ANSWERSTo create a well or pockets in the gel where samples can later be placed. 
 
Why is ethidium bromide added at this step? - ANSWERSEthidium Bromide is needed to see the DNA bands in the gel under UV illumination. 
 
Why is it important to pour slowly and avoid air bubbles? - ANSWERSSuch bubbles would interfere with the movement of the sample through the gel, distorting the results. 
 
What could happen if you do not get a new tip to load each s...
Enrolled Agent Exam Prep - Part 2 Questions & Answers
Which form of business entity is not a legal entity separate and apart from its owner? 
 
A. Corporation 
B. Partnership 
C. Sole proprietorship 
D. S corporation - ANSWERC 
 
Under which circumstance is an entity required to apply for a new employer identification number? 
 
A. Proprietorship that incorporates 
B. Business name change 
C. Electing or revoking S corp status 
D. 50% change of interest in partnership within 12 months. - ANSWERA 
 
LLC's may be an attractive small business alterna...
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Which form of business entity is not a legal entity separate and apart from its owner? 
 
A. Corporation 
B. Partnership 
C. Sole proprietorship 
D. S corporation - ANSWERC 
 
Under which circumstance is an entity required to apply for a new employer identification number? 
 
A. Proprietorship that incorporates 
B. Business name change 
C. Electing or revoking S corp status 
D. 50% change of interest in partnership within 12 months. - ANSWERA 
 
LLC's may be an attractive small business alterna...
Chapter 2 - Practice Before The IRS Questions & Answers
How long should a preparer keep written consents due to conflict of interest - ANSWER36 Months 
 
Where are the proceedings for disbarment of an enrolled agent held? - ANSWERAt a time and place set by an Administrative Law Judge. 
 
If a peparer takes an Annual Federal Tax Refresher (AFTR) course from an approved IRS Continuing Education Provider. Does it count as CE credits towards an EA or AFSP - ANSWERNo, Due to the high level of knowledge required of Enrolled Agents (EAs), this basic level ...
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How long should a preparer keep written consents due to conflict of interest - ANSWER36 Months 
 
Where are the proceedings for disbarment of an enrolled agent held? - ANSWERAt a time and place set by an Administrative Law Judge. 
 
If a peparer takes an Annual Federal Tax Refresher (AFTR) course from an approved IRS Continuing Education Provider. Does it count as CE credits towards an EA or AFSP - ANSWERNo, Due to the high level of knowledge required of Enrolled Agents (EAs), this basic level ...
IRS Enrolled Agent, Part 2, Unit 2 Questions & Answers
Gross Income - ANSWERall income from trade or business 
 
Recovery of previously deducted bad debt expense - ANSWERincluded in gross income to the extent that prior deduction reduced taxes 
 
prepaid income - ANSWER- generally included in year of receipt 
- accrual basis can defer recognition until time of performance for up to 1 year 
 
discharge of debt - ANSWER- generally recognized as income 
- not recognized to extent the payment would've been a deducted 
 
self employment income - ANSWERn...
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Gross Income - ANSWERall income from trade or business 
 
Recovery of previously deducted bad debt expense - ANSWERincluded in gross income to the extent that prior deduction reduced taxes 
 
prepaid income - ANSWER- generally included in year of receipt 
- accrual basis can defer recognition until time of performance for up to 1 year 
 
discharge of debt - ANSWER- generally recognized as income 
- not recognized to extent the payment would've been a deducted 
 
self employment income - ANSWERn...
EA Unit 2 Questions & Answers
basis - ANSWERAB of property given 
+ Gain recognized 
+ Boot given (cash, liability incurred, other property) 
- Boot received (cash, liability relief, other property) + Exchange fees incurred 
- Loss recognized (boot given) 
___________________________________________________ 
= Basis in acquired property 
 
IRS consent is not required for the following changes: - ANSWERAdopting LIFO inventory valuation 
 
Switching to LIFO inventory requires IRS consent. Form 970, Application to Use LIFO Inve...
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basis - ANSWERAB of property given 
+ Gain recognized 
+ Boot given (cash, liability incurred, other property) 
- Boot received (cash, liability relief, other property) + Exchange fees incurred 
- Loss recognized (boot given) 
___________________________________________________ 
= Basis in acquired property 
 
IRS consent is not required for the following changes: - ANSWERAdopting LIFO inventory valuation 
 
Switching to LIFO inventory requires IRS consent. Form 970, Application to Use LIFO Inve...
IRS EA Exam 3 – Representation Questions & AnswersIRS
Taxpayer Advocate Service - ANSWERA taxpayer can request Taxpayer Advocate Service Assistance on Form 911 and must indicate one or more of the following reasons: 
 
-he is experiencing economic harm or is about to suffer economic harm 
-he is facing an immediate threat of adverse action 
-he will suffer irreparable injury or long-term adverse impact if relief is not granted 
-he will incur significant costs of relief is not granted (including fees for professional representation) 
-he has experi...
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Taxpayer Advocate Service - ANSWERA taxpayer can request Taxpayer Advocate Service Assistance on Form 911 and must indicate one or more of the following reasons: 
 
-he is experiencing economic harm or is about to suffer economic harm 
-he is facing an immediate threat of adverse action 
-he will suffer irreparable injury or long-term adverse impact if relief is not granted 
-he will incur significant costs of relief is not granted (including fees for professional representation) 
-he has experi...
Taxation Chapter 2 Questions & Answers
Discriminant Function System	Assigns a score to each tax return that represents the probability the tax liability on the return has been underreported 
Document Perfection Program	A program under which all tax returns are checked for mathematical and tax calculation errors. 
Information Matching Program	A program that compares the taxpayer's tax return to information submitted to the IRS from other taxpayers 
Correspondence Examinations	Most common audit 
Conducted by mail and are generally lim...
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Discriminant Function System	Assigns a score to each tax return that represents the probability the tax liability on the return has been underreported 
Document Perfection Program	A program under which all tax returns are checked for mathematical and tax calculation errors. 
Information Matching Program	A program that compares the taxpayer's tax return to information submitted to the IRS from other taxpayers 
Correspondence Examinations	Most common audit 
Conducted by mail and are generally lim...
EA: Part 3 EX2 taxpayer Questions & Answers
1. Daniella is a tax professional who prepares hundreds of tax returns each year. As such, she is subject to the e-file mandate. She has a new client, Fernando, who refuses to e-file his tax return. What is the proper action for Daniella to take in this case? 
 
A. If her client refuses to e-file, Daniella must decline the engagement. 
B. She must refer Fernando to a different tax return preparer. 
C. She can prepare Fernando's return on paper and attach Form 8948. 
D. She should encourage Fe...
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1. Daniella is a tax professional who prepares hundreds of tax returns each year. As such, she is subject to the e-file mandate. She has a new client, Fernando, who refuses to e-file his tax return. What is the proper action for Daniella to take in this case? 
 
A. If her client refuses to e-file, Daniella must decline the engagement. 
B. She must refer Fernando to a different tax return preparer. 
C. She can prepare Fernando's return on paper and attach Form 8948. 
D. She should encourage Fe...