Bio 250 Quiz with 100% Correct Solutions

Microbiology - study of microbes Fluorescence - visualization of fluorescent molecules that become excited under UV light Direct immunofluorescence - fluorescent stains Transmission electron microscope - visualization of internal structures; electrons are transmitted through specimen Scanning electron microscope - 3D image of specimen; electrons bounce off surface of specimen (gold dust) Macronutrients - needed in large amounts; organic compounds Micronutrients - needed in small amounts; inorganic compounds, trace elements Inoculum - material used to start a culture Culture - Growth of organisms in or on nutrient medium in your select medium Culture medium - broth (liquid), agar (sugars purified from beans that give solid shape); Combination of nutrients used to cultivate microbes Subculture - To make a second culture from an isolated colony Pure culture - A culture that contains only one known organism Mixed culture - A culture that contains two or more known organisms Hypotonic - higher concentrations of solutes in the cell, lower concentration of water in the cell; than in the environment; cells burst Isotonic - equal number of water and solutes inside and outside the cell Hypertonic - higher concentrations of solutes and water outside of the cell than in the environment; cells shrink Nucleus - provides protection/transcription