Gel Electrophoresis Questions and Answers Already Passed
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Course
Gel Electrophoresis
Institution
Gel Electrophoresis
Gel Electrophoresis Questions and
Answers Already Passed
What property of DNA allows it to migrate towards the positive electrode during gel
electrophoresis?
DNA is negatively charged due to its phosphate backbone, causing it to move towards the
positive electrode when an electric field is...
Gel Electrophoresis Questions and
Answers Already Passed
What property of DNA allows it to migrate towards the positive electrode during gel
electrophoresis?
✔✔DNA is negatively charged due to its phosphate backbone, causing it to move towards the
positive electrode when an electric field is applied.
In gel electrophoresis, what role does the gel matrix play in the separation of molecules?
✔✔The gel matrix acts as a sieve, allowing smaller molecules to pass through more easily while
restricting the movement of larger molecules.
What alternative to agarose gel can be used for separating proteins in electrophoresis?
✔✔Polyacrylamide gel is often used for separating proteins, providing higher resolution than
agarose for small protein sizes.
How does the voltage applied during gel electrophoresis influence the separation of DNA
fragments?
✔✔Higher voltages can speed up the migration of DNA fragments but may also cause increased
heating, which can affect resolution and cause band distortion.
1
, What is the effect of adding SDS (sodium dodecyl sulfate) in protein gel electrophoresis?
✔✔SDS denatures proteins and imparts a uniform negative charge, allowing separation based on
molecular weight rather than shape or charge.
Why is it essential to run a control sample alongside experimental samples in gel
electrophoresis?
✔✔Running a control sample helps verify the experimental conditions and ensures that results
are reliable and interpretable.
How can gel electrophoresis be applied in gene therapy research?
✔✔Gel electrophoresis can be used to confirm the presence and size of therapeutic gene
constructs before they are introduced into patient cells.
What are some common staining methods used for visualizing nucleic acids after gel
electrophoresis?
✔✔Common staining methods include ethidium bromide staining, SYBR Green, and GelRed, all
of which bind to DNA and fluoresce under UV light.
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