Pcr product - Samenvattingen, Aantekeningen en Examens

ASCP Exam practice Toolkit Questions with A+ rated answers 2023/2024 E. coli RNA Polymerase II - correct answer DNA template, product: mRNA RNA Polymerase I - correct answer DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - correct answer DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III - correct answer DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase - correct answer DNA temp...

WGU C785 Biochemistry FINAL EXAM Questions With Correct Answers Latest 2024 (GRADED) What is the basic structure of an amino acid? What do they look like? - Answer amino group (NH2 or NH3), carboxyl group (COO or COOH), alpha carbon (C), and variable group How do you identify the 3 different types of side chains: non-polar/hydrophobic, polar, and charged? - Answer Non-polar/hydrophobic - end with CH or "can't have" water. Polar - end with OH, SH, or NH. Charged - end with a charge what ...

Where is cortisol released from correct answers adrenal cortex An adipocyte contains: correct answers triglycerides and hormone receptors on the cell membrane The structural back bone of a steroid hormone is correct answers Cholesterol In an oxidation reduction reactions the molecule that is the REDUCING agent is: correct answers The molecule that donates an electron In an oxidation reduction reaction the molecule that is the OXIDIZING agent is: correct answers The molecule that acce...

In dit Labjournaal van de cursus Practicum Moleculaire Biologie (PMOB) zijn alle lessen volledig uitgewerkt. Inclusief voorbeeld berekeningen, pipetteerschema's, resultaten, conclusies en discussies. Je kunt dit gebruiken als voorbeeld, niet om volledig te kopiëren. Controleer ook alles zelf nog voordat je het inlevert. Succes!

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

physical fitness - Answer- the ability to perform moderate to vigorous activity without undue fatigue ATP (adenosine triphosphate) - Answer- the main energy currency for cells. Used to promote ion pumping, enzyme activity, and muscular contraction. The immediate source of energy for body functions. ADP (adenosine diphosphate) - Answer- A breakdown product of ATP. Synthesized into ATP using energy from foodstuffs and a phosphate group. phosphocreatine (PCr) - Answer- A high-energy compou...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

Catalyst - ANSWER-a substance that increases the rate of a chemical reaction without being consumed Enzyme - ANSWER-a molecule that increases the rate of a chemical reaction without being consumed (ex: protein) Activation energy - ANSWER-amount of energy required to convert a substrate into a product Active site - ANSWER-location on an enzyme that physically interacts with the substrate How do enzymes catalyze reactions? - ANSWER-1. substrate attaches to active site 2. activation en...

d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) - Answer *A DNA sequence with high GC content is more likel...

The success of a PCR reaction is mostly dependent on what? - well designed primers Primer design can also be applied to what? - - probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - - Primer length - Percent GC content - Melting temperature - Sequence How are Primer...