Lineweaver - Samenvattingen, Aantekeningen en Examens

Dit document omvat een oefententamen voor het vak werking van geneesmiddelen (BA-104) voor de studie farmacie aan de universiteit Utrecht. Het oefententamen bevat in totaal 6 vragen (met subvragen a,b,c,d etc). Er is geprobeerd om het oefententamen zo dicht mogelijk bij het niveau van het echte tentamen te brengen. Onderwerpen die aan bod komen zijn o.a. signaaltransductieroutes, tekenen van een scatchardplot en lineweaver-burkplot. Ook komen de onderwerpen: gentranscriptie, agonisten, antagonis...

(T/F) Acid-base catalysis involving free protons or hydroxide ions is thermodynamically favorable because these are already in high concentrations in the cell. Correct Answer-False (T/F) The glycerophosopholipids of a membrane bilayer have been modified to contain additional double bonds. This change will likely lower the activation of adenylate cyclase by a G-protein. Correct Answer-False (T/F) To be inserted, a α-helical integral membrane protein requires at least one helix that is at l...

Dr Rai biochem test 2 questions & answers 2023/2024 kinetic mechanism - ANSWER-the order of binding of substrates and release of products sequential mechanism - ANSWER-each substrate binds but both substrates must be bound by enzyme to form a ternary complex before catalysis can occur ping pong mechanism (double displacement) - ANSWER-Substrate binds to the enzyme and releases a product before the second substrate binds to the enzyme Random order enzyme mechanism - ANSWER-any react...

Biochemistry NBME Exam 2024 Well Revised and Verified Answers Updated Proteoglycans Act as molecular sieves in ECM, CT, and cartilage GAGs are the long polysacc chains attached to core protein GAG is heavily hydrated and cushions (has "-" charge) Degraded via lysosomal pathway (defect = MPS) Glycolipids A.K.A. sphingolipids Made with ceramide lipid base Serine + palmitoyl-CoA => sphingosine (+FFA) => ceramide Carb part sticks out into ECM (same w/ glycoproteins) Side chain p...

Units 1-5 - Memorize all 20 amino acids - Memorize what all the complex carbs look like and a few facts about them - Know Michaleis-Menton graph and be able to identify type of enzyme inhibitor - Be able to explain how to get Vmax and Km from Lineweaver-Burk - Know how to translate DNA to mRNA and know the peptide that would be translated o do not need to memorize codons - Outline steps of translation - Know about all categories of: o Lipids o Active/passive transport o Plasmids o Re...

diffusion controlled limit - Answer The theoretical maximum rate of an enzymatic reaction in solution, about 10^8 to 10^9 M^-1 * s^-1 catalytic perfection - Answer A state achieved by an enzyme that operates at the diffusion-controlled limit. Lineweaver-Burk plot - Answer A rearrangement of the michaelis menton equation that permits the determination of KM and Vmax from a linear plot.

Biochemistry NBME Exam 2024 Well Revised and Verified Answers Updated Proteoglycans Act as molecular sieves in ECM, CT, and cartilage GAGs are the long polysacc chains attached to core protein GAG is heavily hydrated and cushions (has "-" charge) Degraded via lysosomal pathway (defect = MPS) Glycolipids A.K.A. sphingolipids Made with ceramide lipid base Serine + palmitoyl-CoA => sphingosine (+FFA) => ceramide Carb part sticks out into ECM (same w/ glycoproteins) Side chain p...

BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS Unfolds proteins and gives them uniform negative charge. - answerSDS Reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids - answerFDNB Reduces disulfide bonds. - answerDTT Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding. - answerIodoacetate Bonds between two -SH groups that form between 2°...

BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS Unfolds proteins and gives them uniform negative charge. - answerSDS Reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids - answerFDNB Reduces disulfide bonds. - answerDTT Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding. - answerIodoacetate Bonds between two -SH groups that form between 2°...

Which of the following interactions are generally considered to be unfavorable? - Interaction between Arg and Lys side chains at a pH of 7, assuming standard pKa values for Arg and Lys Proteins can be denatured by a) Changing temperature b) Adding urea to solution c) Changing solvent d) all choices are correct - All choices are correct If PAGE is carried out after subjecting the sample to SDS and beta-mercaptoethanol, then proteins will be separated on the basis of - Mass Which is the most...