UTA Micro Lab Practical One Review (Quizzes) Latest Update Graded A+

UTA Micro Lab Practical One Review (Quizzes) Latest Update Graded A+ If you are using the 40x objective and have a 10x eyepiece, what is your total magnification? 400x __________ is the ability to see or distinguish detail or the clarity of the image. Resolution What can be done to increase the contrast of a specimen to bright background of a light microscope? You could stain the specimen. Based off of your lab activities, what is this image of? bacterial capsule When using a parfocal lenses, coarse adjustment is typically only done when the specimen is initially viewed using the ______ objective. 4x Based off of your lab activities, what magnification is this image? 100x What is the purpose of using immersion oil with the 100X objective? To reduce the scattering or bending of light from the image. What magnification is this onion root tip image based off of your lab activity? 40x _________ is a measure of how much the size of the size of the image of the specimen has been increased. Magnification It is ok to use oil on the 40x objective to reduce light scattering. T/F False Which statement below is correct? Allow the loop to cool slightly before putting it into a culture by waiting a few seconds before inoculating. Jeffery inoculated a tryptone broth tube with E. coli. When he examined his broth tube during the next lab period, there was no growth at all. What could have gone wrong with his experiment? All Correct: o Jeffrey may not have allowed his loop to cool adequately. o The culture could possibly have been dead. o He could have not actually removed any inoculum (missed dipping his loop into the broth). For proper aseptic technique, you should flame the opening of the tube after you remove the cap and before you put the cap back on. T/F True The loop should be sterilized in what part of the Bunsen burner flame? Just above the bright blue cone Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer? little finger of the dominant hand (hand holding the loop) What tool is used when transferring organisms from a liquid medium? Loop The two most common instruments to transfer inoculum