Class notes
Fluorescence Spectroscopy
- Institution
- University Of Limpopo (UL)
third level Fluorescence Spectroscopy study notes
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MOLECULAR FLUORESCENCESPECTROSCOPY
1
,What is Fluorescence Spectroscopy
• Fluorescence is a type of luminescence caused by
photons exciting a molecule, raising it to an electronic
excited state.
• Fluorescence spectroscopy uses a beam of light that
excites the electrons in molecules of certain compounds,
and causes them to emit light. That light is directed
towards a filter and onto a detector for measurement and
identification of the molecule or changes in the molecule.
2
, Fluorophores
• Fluorophores :
• A sample that is able to fluoresce
• can absorb and emit light within a range of
wavelengths, normally referred to as the
absorbance (excitation) and emission
spectra.
3
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