Some examples from this set of practice questions
1.
How are bacteria quantified in a mixed culture?
Answer: CFUs x dilution factor x volume (in ml)
2.
How is motility determined?
Answer: Using a wet mount and viewing under a microscope.
3.
What is Brownian movement?
Answer: Results from the random motion of water molecules surrounding the bacteria.
4.
What are the 3 types of motility?
Answer: Flagellar, spiral, gliding
5.
How is cell morphology determined?
Answer: Viewing under a microscope.
6.
What is colony morphology?
Answer: The colonies as seen on an agar plate — shape, edge, surface, elevation
7.
What is the purpose of a gram stain?
Answer: Used to differentiate between gram-positive, gram-negative and non-staining bacteria.
8.
What colour do gram-positive bacteria stain?
Answer: Purple, as they retain the crystal violet (thicker peptidoglycan layer).
9.
What colour do gram-negative bacteria stain?
Answer: Pink, as they do NOT retain the crystal violet.
10.
How are indistinct gram stain results confirmed?
Answer: Using KOH, where gram-negative bacteria produce a mucoid string, while gram-positive bacteria remain liquid.
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