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Summary Basic Introductory Histology Notes R50,00
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  3. University Of The Witwatersrand (wits)
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  5. Anatomy (ANAT2021)

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Summary Basic Introductory Histology Notes

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A detailed introduction to provide a foundation into understanding histology and histological components. These notes are a basic summary of histology to grasp the fundamental key elements of this topic.

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  • Chapter 1 to chapter 3
  • December 2, 2021
  • 19
  • 2021/2022
  • Summary

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DEFINING HISTOLOGY

• Histology- Study of tissues and arrangement into organs
• Tissue = cells + extracellular matrix (ECM)
▪ Cells
– Major compartments = nucleus & cytoplasm(with organelles)
▪ ECM
– Structural network surrounding cells
– Influences cellular communication and function
– Contain fibres (collagen/elastic) and ground substance (proteoglycans, glycoproteins and
glycosaminoglycans)
• Structure is related to function!

HISTOLOGICAL TECHNIQUES

→ The routinely prepared hematoxylin and eosin–stained
section is the specimen most commonly studied.
• Haematoxylin
– Basic dye
– Stains acidic structures basophilic
– Colour: purple/dark blue
– Basic dyes react with anionic components of cells and tissue (components that carry a net negative
charge).
– A basic dye carries a net positive charge on its colored portion and is described by the general
formula [dye+ Cl− ].
– Anionic components include the phosphate groups of nucleic acids, the sulfate groups of
glycosaminoglycans, and the carboxyl groups of proteins.
– The ability of such anionic groups to react with a basic dye is called basophilia
– Hematoxylin is not, strictly speaking, a basic dye. It is used with a mordant (i.e., an intermediate link
between the tissue component and the dye).
– Tissue components that stain with hematoxylin also exhibit basophilia.
• Eosin
– Acidic dye
– Stains acidic structures eosinophilic
– Colour: pink
– An acidic dye carries a net negative charge on its colored portion and is described by the general
formula [Na+ dye−].
– Acidic dyes react with cationic groups in cells and tissues,
particularly with the ionized amino groups of proteins.
– The reaction of cationic groups with an acidic dye is
called acidophilia.
– Reactions of cell and tissue components with acidic
dyes are neither as specific nor as precise as reactions
with basic dyes

,→ A limited number of substances within cells and the extracellular matrix display basophilia.
• heterochromatin and nucleoli of the nucleus (chiefly because of ionized phosphate groups in nucleic
acids of both)
• cytoplasmic components such as the ergastoplasm (also because of ionized phosphate groups in
ribosomal RNA)
• extracellular materials such as the complex carbohydrates of the matrix of cartilage (because of
ionized sulfate groups).

→ Staining with acidic dyes is less specific, but more substances within cells and the extracellular matrix
exhibit acidophilia.
• most cytoplasmic filaments, especially those of muscle cells
• most intracellular membranous components and much of the otherwise unspecialized cytoplasm
• most extracellular fibers (primarily because of ionized amino groups).




→ Fixation, usually by a chemical or mixture of chemicals, permanently preserves the tissue structure for
subsequent treatments.
→ Specimens should be immersed in fixative immediately after they are removed from the body.
→ Fixation is used to:
• terminate cell metabolism,
• prevent enzymatic degradation of cells and tissues by autolysis (self-digestion),
• kill pathogenic microorganisms such as bacteria, fungi, and viruses
• harden the tissue as a result of either cross-linking or denaturing protein molecules.




Hematoxylin staining Eosin staining Hematoxylin & Eosin staining

, MICROSCOPY

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