Summary
Summary Genetics 244 Ch20 notes: Recombinant DNA Technology
- Course
- Genetics 244
- Institution
- Stellenbosch University (SUN)
These notes include a full summary of chapter 20 in the textbook, as well as notes taken in lectures.
[Show more]
Connected book
Book Title:
Author(s):
- Edition:
- ISBN:
- Edition:
Seller
Follow
Content preview
Genetics 244Chapter 20: Recombinant DNA Technology
Lecture 1:
- Also called genetic engineering
- Based on cutting and pasting DNA molecules at
specific base sequences
- The resulting fragment can be transferred from any
organism or virus into a bacterial cell
- The DNA fragment can be mass-produced, isolated
and characterized in the bacterial cell
Uses:
- Gene mapping
- Diagnosis of disease
- Commercial production of human gene products
- Expression of foreign genes in transgenic animals
and plants
- The first commercial “biotech product” was insulin
in 1982
Making recombinant DNA:
- The process is made up of several steps
- These steps are universally used for all
applications
1. DNA is isolated from cells
o Find a way to isolate the specific sequence you
are interested in
2. DNA is cut by restriction enzymes (REs)
3. DNA fragments are ligated (pasted) into vectors
, o Using ligases which fix phosphodiester bonds
4. Recombinant molecules are transformed into host
cells
5. Multiplication occurs in the host cells
6. Cloned DNA can be recovered, purified and
analyzed
Restriction enzymes:
- “Molecular scissors”
- A tool used in recombinant technology
- There is a defense mechanism of bacteria against
invading phages
- Endonucleases (REs) cut and inactivate the DNA of
the phage, allowing the phage to invade the
bacterial host cell
- Type II REs recognize and cut at the exact place
- Palindromic recognition sequences (3’ and 5’ are
the same sequence backwards)
- Generate restriction fragments
Frequent vs rare cutters
- Frequent cutters recognize six-base sequences
- 46= 4096bp
- Rare cutters recognize four-base sequences
, - 44= 356bp
- Both frequent and rare cutters always recognize
palindromic sequences
Sticky vs blunt ends
- Sticky (cohesive) ends cut with overhang
- Recognize 3’ vs 5’ overhangs
- Overhangs are complementary and can be fixed by
ligase
- Blunt ends do not have an overhang
- They are difficult to ligate because there are no
complementary base pairs to fix
Creating a recombinant DNA molecule
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through EFT, credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying this summary from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller paytonkilgour. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy this summary for R80,00. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67866 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy summaries for 14 years now
