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Samenvatting Practicum histologie en histopathologie - volledige uitwerking preparaten

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  • Course
  • Histologie en histopathologie van de orgaanstelsels
  • Institution
  • Universiteit Antwerpen (UA)

In dit document vindt je elk preparaat terug dat tijdens de practica wordt besproken. Ze zijn voorzien van extra uitleg bij de structuren en aanduidingen op de preparaten. De ideale aanvulling om het praktijk gedeelte van het vak tot een goed einde te brengen!

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Document information

  • June 19, 2024
  • 103
  • 2022/2023
  • Summary

Subjects

  • histologie
  • histopatho
  • histopathologie
  • pathologie
  • practicum
  • microscopie

Written for

  • Universiteit Antwerpen (UA)
  • Biomedische Wetenschappen
  • Histologie en histopathologie van de orgaanstelsels
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HISTOLOGIE EN
HISTOPATHOLOGIE
(PRACTICUM)
2e Ba Biomedische Wetenschappen




Universiteit Antwerpen

,Inhoudsopgave
practicum 1: circulatiestelsel en lymfoïde organen .......................................................................................... 4
histologie – circulatiestelsel ................................................................................................................................. 4
I 3/9: A+V+N Femoralis .................................................................................................................................... 4
I 4/3: aorta ....................................................................................................................................................... 5
I 3/7 arteria muscularis .................................................................................................................................... 6
I 1/7: vena porta .............................................................................................................................................. 7
F 2/9: ureter ..................................................................................................................................................... 7
E 12/3: dunne darm ......................................................................................................................................... 8
I 5/22: hart ....................................................................................................................................................... 8
Histologie – lymfoïde organen ............................................................................................................................. 9
C 2/4: beenmerg .............................................................................................................................................. 9
J 4/13: thymus................................................................................................................................................ 10
J 1/11: lymfeknoop ........................................................................................................................................ 11
J 2/10: tonsilla pallatina ................................................................................................................................. 12
J 3/6: milt ....................................................................................................................................................... 13
E 14/21: ileum (platen van peyer) ................................................................................................................. 14

practicum 2: Nier en urinewegen ................................................................................................................... 14
F 1/44: nier – konijn ....................................................................................................................................... 14
F 1/42: nier – humaan .................................................................................................................................... 16
F 1/3: nier – muis ........................................................................................................................................... 17
F 1/43: nier – rat ............................................................................................................................................ 17
F 2/11: ureter ................................................................................................................................................. 18
F 3/13: vesica urinaria (urineblaas) ................................................................................................................ 19

Practicum 3: Pathologie circulatiestelsel, lymfoïde organen, nier & urinewegen ........................................... 19
IP 4/1: arteria coronaria met atherosclerose (slagaderverkalking) en thrombose ........................................ 19
IP 5/1: hart- myocard ischemie ...................................................................................................................... 21
bloedvattumor – huid met angioom .............................................................................................................. 22
JP 1/1: lymfeknoop met sarcoïdose ............................................................................................................... 22
JP 3/1: milt – hemosiderose ........................................................................................................................... 23
JP 3/2: milt – lymfoom – hond ....................................................................................................................... 24
CP 2/1: beenmerg – ziekte van gaucher – humaan ....................................................................................... 24
FP 1/2: nier met papillair renaal carcinoom .................................................................................................. 25
FP 1/1: nier met congenitale dysplasie en chronische ontsteking – hond .................................................... 25
FP 3/1: urineblaas – vorming van granulatieweesel – humaan ..................................................................... 26

practicum 4: ademhalingsstelsel .................................................................................................................... 27
L1/1,2: septum nasi........................................................................................................................................ 27
L4/3: larynx .................................................................................................................................................... 28
L5/2: pharynx ................................................................................................................................................. 29
L2/11: trachea ................................................................................................................................................ 30
l3/29: long – varken ....................................................................................................................................... 31
L3/26: long – rat ............................................................................................................................................. 33

practicum 5: pathologie ademhalingsstelsel................................................................................................... 35
LP 1/1: neus – papilloma – humaan ............................................................................................................... 35
LP 4/1: larynx – plaveiselepitheelcarcinoom – humaan ................................................................................ 36
lp 3/1: long – infarct- humaan ....................................................................................................................... 37
LP 3/2: long – acute longontsteking (pneumonie) – humaan ........................................................................ 38
lp 3/3: long – chronische pneumonie – humaan ........................................................................................... 39



1

, LP 3/4: pleuraholte – empyeem in de pleuraholte – humaan ....................................................................... 40
LP 3/5: long – ongedifferentieerd grootcellig carcinoma – humaan ............................................................. 41
LP 3/6: long – kleincellig longcarcinoom van de bronchus – humaan ........................................................... 42
lp 3/7: organiserende acute bacteriële pneumonie – humaan ..................................................................... 44
LP 3/8: aspergillose – humaan ....................................................................................................................... 45

practicum 6: Histologie – spijsvertering van mondholte tot maag .................................................................. 45
E1/5: lip – humaan – Van Gieson ................................................................................................................... 45
E4/13: tand – varken – azan .......................................................................................................................... 47
E7/39: Glandula submandibularis – humaan – trichroom, masson ............................................................... 49
E5/34: tong – hond – he ................................................................................................................................ 50
E5/35: tong met papillae circumvallatae – hond – he ................................................................................... 52
E8/15: oesophagus – humaan – trichroom, masson ..................................................................................... 53
E10/18: fundus – varken – he ........................................................................................................................ 54
E11/11: pylorus – varken – azan .................................................................................................................... 55

practicum 7: Histologie spijsvertering: darmkanaal + lever; galblaas en pancreas .......................................... 56
E11/10: overgang pylorus-duodenum – rat- PAS heamatoxiline ................................................................... 56
E12/9: jejunum – rat – azan ........................................................................................................................... 58
E14/20: ileum – varken – azan ....................................................................................................................... 58
E14/22: dunne darm – humaan – trichroom, masson ................................................................................... 59
E15/1,2: colon met taenia – humaan – azan ................................................................................................. 60
E16/8: appendix – humaan – trichroom ........................................................................................................ 61
E17/3: overgang rectum-anus – HE ............................................................................................................... 62
E18/48: lever – varken – trichroom, lichtgroen ............................................................................................. 63
E18/54: lever – humaan – trichroom masson ................................................................................................ 65
E18/15: lever – hond – zilverimpregnatie ...................................................................................................... 66
E18/52: lever – rat – trypaanblauw, kernechtrood ....................................................................................... 67
E18/53: lever – rat – ATPase .......................................................................................................................... 67
E19/5: galblaas – hond – azan ....................................................................................................................... 68
E20/26: pancreas – rat – trichroom ............................................................................................................... 69

practicum 8: histopathologie van het spijsverteringsstelsel ........................................................................... 70
EP7/1: speekselklier – mengtumor in de parotisklier – humaan – he ........................................................... 70
EP15/1: colon – adenocarcinoom – humaan – HES ....................................................................................... 71
EP15/2: colon – colitis ulcerosa – humaan – he ............................................................................................ 73
EP16/1: appendix – acute appendicitis – humaan – he ................................................................................. 73
EP18/1: lever – steatose – humaan – he ....................................................................................................... 74
EP18/3: lever – cirrhose – hond – he ............................................................................................................. 75
EP18/2: lever – amyloïdose – humaan – congorood ..................................................................................... 76
EP18/4: lever – hemangioom – humaan – he ................................................................................................ 76
EP20/1: pancreas – acute pancreatitis – hond – he....................................................................................... 77
ep 20/2: pancreas – chronische pancreatitis – kat – he ................................................................................ 77

practicum 9: endocriene klieren + mannelijk en vrouwelijk voortplantingsstelsel .......................................... 78
endocriene klieren ............................................................................................................................................. 78
E20/26: pancreas – rat – trichroom ............................................................................................................... 78
K1/35: glandula suprarenalis (bijnier) – rat – trichroom, lichtgroen ............................................................. 78
K2/17: glandula thyroidea – varken – azan (BW blauw aangekleurd) ................................... 80
K3/2: glandula parthyroidea – humaan – he ................................................................................................. 82
K1/5: hypophysis – humaan – he ................................................................................................................... 82
K5/17: hypofyse – mens – HE ........................................................................................................................ 84
mannelijk voortplantingsstelsel ......................................................................................................................... 85
G1/17: testis – kat – azan............................................................................................................................... 85



2

, G6/12: prostaat – kat – azan.......................................................................................................................... 86
G7/3: penis – kat – azan ................................................................................................................................. 88
vrouwelijk voortplantingsstelsel ........................................................................................................................ 89
H1/50: ovarium – aap – azan ......................................................................................................................... 89
H4/13: uterus – aap – azan ............................................................................................................................ 90
D15/11: bortstklier – trichroom masson met heidenhainsblauw .................................................................. 92

practicum 10: pathologie endocriene klieren + mannelijk en vrouwelijk voortplantingsstelsel ...................... 93
endocriene klieren ............................................................................................................................................. 93
KP2/1: glandula thyroidea – multinodulaire goiter – humaan ...................................................................... 93
mannelijk en vrouwelijk voortplantingsstelsel ................................................................................................... 95
GP6/1: prostaat – benigne hyperplasie – humaan – hes ............................................................................... 95
HP1/1: ovarium – teratoom – humaan – hes ................................................................................................ 96
HP4/1: uterus – leiomyoom – humaan – hes ................................................................................................ 98
D 15/11: borstklier – metaplasie.................................................................................................................... 99
DP15/1: borstklier – fibroadenoom – humaan – hes ................................................................................... 100
DP15/2: borstklier – carcinoom – humaan – hes ......................................................................................... 101




3

,PRACTICUM 1: CIRCULATIESTELSEL EN LYMFOÏDE ORGANEN
HISTOLOGIE – CIRCULATIESTELSEL

I 3/9: A+V+N FEMORALIS

ALGEMENE BOUWPLAN BLOEDVATEN

Tunica intima (kernen, subendotheliaal BW en membrana elastica interna)
- Laag endotheelcellen
- Subendotheliaal bindweefsel: losmazig en beperkt aanwezig (soms gladde spiercellen)
- arteriën: membrana elastica interna tussen intima en media → versmelting longitudinaal georiënteerde
elastische vezels

Tunica media (membrana elastica externa en vezels)
- Circulair gerangschikte gladde spiercellen, met ertussen wisselende hoeveelheid elastische en collagene vezels
- Membrana elastica externa tussen media en adventitia: vezelig en minder scherp afgelijnd

Tunica adventitia
- Hoofdzakelijk bindweefsel dat concentrisch gelegen is rond de bloedvaten → veel longitudinaal gericht collageen
en soms gladde spiervezels
- Bij grotere vaten: vasa vasorum, vasa lymphatica vasorum en zenuwen terug te vinden
- Gaat zonder scherpe grens over in omliggende bindweefsel rond bloedvat

Arteria Femoralis


Tunica adventitia
Membrana elastica externa

Tunica media

Membrana elastica interna
(sluit aan op tunica intima)


Vena Femoralis

Tunica adventitia

Tunica media

Membrana elastica interna
(sluit aan op tunica intima)




4

, I 4/3: AORTA

ELASTISCHE ARTERIËN:

Tunica intima
- Dik subendotheliale laag
- Longitudinale bindweefselvezels (elastisch)
- Luminale kant: endotheelcellen → hoge metabole en synthetische activiteit

Tunica media
- Hoog gehalte aan elastine(!)
- Concentrische elastische membranen met openingen en ertussen schuine, korte gladde spiercellen → houden
membranen samen
- Dunne collageen vezels en amorfe tussenstof

Tunica adventitia
- Geen elatische membranen
- Veel longitudinaal verlopende elastische en collagene vezels waartussen kernen van fibroblasten en gladde
spieren zichtbaar zijn
- Soms vasa vasorum → voorzien bloedvaten van bloed




Tunica adventitia



Tunica media



Tunica intima




Kern gladde spiercel

Zwarte elastinevezels
Groen collageen BW

Tunica intima met
endotheelcellen


Subendotheliaal BW




Vasa vasorum




5

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