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Summary Achem11 Chromatographic Methods of Separation

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This is a comprehensive and detailed summary on Chromatographic Methods of Separation for Achem11. Essential!!

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  • August 8, 2024
  • 31
  • 2019/2020
  • Summary

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CHROMATOGRAPHIC
METHODS OF SEPARATION




1

, BASIC PRINCIPLES
• All chromatographic separations rely on the
differences in interaction between analytes
and the two characteristic phases
• Mobile phase: carries/transports the
analytes
• Stationary phase: interacts with the
analytes as they are moving through it.

• Analytes that strongly interact with the
stationary phase are retained longer, thus
elute from the column later than those that
interact weakly with the stationationary
phase.
• Analytes separate into bands

• Analytes are detected at the exit of the
column and their signals recorded

• Plot: chromatogram




2

,• Classification based on the types of mobile and
stationary phases and the kinds of equilibria involved in
the transfer of solutes between phases
• Name based on type of Mobile




3

, Elution
• Elution: washing a
species through a
column by continuous
addition of fresh mobile
phase
• Mobile phase: eluent
• Partition between
mobile and stationary
phase




4

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