Gel Electrophoresis Lab Quiz: Questions & A+ Answers
What direction does the DNA travel? Right Ans - negative to positive
What tool was used to insert the DNA into the gel? Right Ans -
micropippeter
Is DNA positive or negative? Right Ans - negative
What virus was used? Right Ans - lambda virus
Which restriction enzyme was the ladder, or the one we compared all the
other results to? Right Ans - HindIII
What type of gel was used? Right Ans - agarose
What would occur if the voltage used was increased? Right Ans - The DNA
fragments would have travelled faster
What would occur if the voltage used was decreased? Right Ans - The DNA
fragments would have travelled slower
What would occur if the running time (of electrophoresis) was increased?
Right Ans - The DNA fragments would travel farther
What would occur if the running time (of electrophoresis) was decreased?
Right Ans - The DNA fragment would travel slower
What would occur if the amount of DNA used was increased? Right Ans -
The DNA bands would be darker
What would occur if the amount of DNA used was decreased? Right Ans -
The DNA bands would be lighter
What would occur if the polarity was reversed? Right Ans - The bands
would move the opposite way, causing them to fall off the gel and into the
buffer.
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