CMB2001 Questions and Answer | Latest Update 2024/2025
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Course
CMB2001
Institution
CMB2001
What is gene expression?
~~> dsDNA is decoded to ssRNA/proteins by an mRNA intermediate
using A/U/C/GTPs and RNA polymerase
Where is the consensus sequence in prokaryotic promoters?
~~> at -10 and -35 upstream of the the transcription start site
What recognises the consensus sequences...
✓ ~~> can either increase or decrease transcription of genes
depending on which amino acids are methylated, and how many
methyl groups are attached
What does eukaryotic DNA have upstream of the transcription start site?
✓ ~~> upstream regulatory regions
Whats an examples of upstream regulatory regions?
✓ ~~> CAAT box which is an UAS (upstream activating sequences)
Whats an examples of upstream regulatory regions?
✓ ~~> GC box (UAS)
What are UAS/URS?
✓ ~~> upstream activating/repressor sequences without activator
sequences the formation of PIC is ineffective and leads to poor
transcription
A way to identify promoter elements?
Master01 | September, 2024/2025 | Latest update
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