Gel Electrophoresis Questions and Answers Already Passed
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Course
Gel Electrophoresis
Institution
Gel Electrophoresis
Gel Electrophoresis Questions and
Answers Already Passed
What is gel electrophoresis primarily used for in molecular biology?
Gel electrophoresis is primarily used for separating nucleic acids or proteins based on their
size and charge.
How does gel electrophoresis work to separate DNA ...
Gel Electrophoresis Questions and
Answers Already Passed
What is gel electrophoresis primarily used for in molecular biology?
✔✔ Gel electrophoresis is primarily used for separating nucleic acids or proteins based on their
size and charge.
How does gel electrophoresis work to separate DNA fragments?
✔✔ Gel electrophoresis works by applying an electric field to a gel matrix, causing negatively
charged DNA fragments to migrate towards the positive electrode, with smaller fragments
moving faster than larger ones.
What types of gels are commonly used in gel electrophoresis?
✔✔ Agarose and polyacrylamide gels are commonly used, with agarose typically used for DNA
separation and polyacrylamide for proteins.
What role does a loading dye play in gel electrophoresis?
✔✔ A loading dye adds color to the samples, allowing visualization during loading and
monitoring of the migration progress through the gel.
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, How is the gel prepared for electrophoresis?
✔✔ The gel is prepared by mixing agarose or polyacrylamide with a buffer solution, heating it to
dissolve, pouring it into a mold, and allowing it to solidify.
What is the significance of the buffer solution used in gel electrophoresis?
✔✔ The buffer solution maintains a stable pH and provides ions that facilitate the conductivity
of electricity through the gel.
What does the term "molecular weight marker" refer to in gel electrophoresis?
✔✔ A molecular weight marker is a mixture of known DNA or protein fragments used as a
reference to estimate the sizes of unknown samples during electrophoresis.
Why is it important to run a control sample in gel electrophoresis?
✔✔ Running a control sample allows for comparison and helps validate the results, ensuring that
the experimental setup is functioning properly.
How can the results of gel electrophoresis be visualized?
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