Biology- Gel Electrophoresis Questions and Answers Graded A+
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Course
Biology- Gel Electrophoresis
Institution
Biology- Gel Electrophoresis
Biology- Gel Electrophoresis Questions
and Answers Graded A+
How does the electric field strength affect the resolution of a gel?
A stronger electric field increases the speed of migration but can reduce resolution; a
balanced field strength is crucial for optimal separation.
What precau...
Biology- Gel Electrophoresis Questions
and Answers Graded A+
How does the electric field strength affect the resolution of a gel?
✔✔A stronger electric field increases the speed of migration but can reduce resolution; a
balanced field strength is crucial for optimal separation.
What precautions should be taken when using UV light to visualize DNA bands?
✔✔Protective eyewear should be worn, and the exposure time should be minimized to avoid
damaging the DNA and harming the eyes.
What strategies can be employed to improve the separation of closely sized DNA fragments?
✔✔Using a gradient gel or adjusting the gel concentration can enhance the resolution between
closely sized fragments.
How can gel electrophoresis be utilized in environmental DNA (eDNA) analysis?
✔✔It allows for the identification of species presence in environmental samples by analyzing
DNA extracted from soil or water samples.
1
, What are some common staining methods used to visualize DNA after electrophoresis?
✔✔Ethidium bromide, SYBR Safe, and GelRed are common stains used to visualize DNA under
UV light after gel electrophoresis.
What factors influence the choice of agarose concentration in gel electrophoresis?
✔✔The size of the DNA fragments to be separated influences agarose concentration; lower
concentrations are used for larger fragments, while higher concentrations are suited for smaller
ones.
How does the presence of charge affect the migration of molecules in gel electrophoresis?
✔✔Molecules with a negative charge move towards the positive electrode, while positively
charged molecules migrate towards the negative electrode, influencing their separation based on
charge.
What is the significance of using a tracking dye in gel electrophoresis?
✔✔A tracking dye provides a visual reference to monitor the progress of the electrophoresis run,
helping to avoid over-running the gel.
How can gel electrophoresis be used in the analysis of RNA?
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