A cerebrospinal fluid (CSF) pattern for hematologic evaluation must be examined within what
number of hours of collection?
1 hour
2 hours
3 hours
four hours - ANS-1 hour
A DNA specimen turned into despatched to your Molecular Diagnostics Laboratory for DNA
sequencing, to be able to decide whether affected person A has a selected mutation in gene X.
The gene sequence is thought. However, the gene mutation itself isn't known. Therefore, DNA
sequencing can not be accomplished on this DNA specimen. (True or False)
True
False - ANS-True
A determine has an autosomal dominant sickness. What percentage danger does this discern
have to skip down this affected gene to his/her infant?
0%
25%
50%
75%
one hundred% - ANS-50%
A determine has an autosomal recessive ailment. What percent danger does this figure must
bypass down this affected gene to his/her baby?
Zero%
25%
50%
seventy five%
a hundred% - ANS-a hundred%
A patient with impaired judgment, character modifications, signs and symptoms of odd frame
actions and despair comes to the health practitioner's office for a follow-up go to. The doctor
suspects a single-gene ailment may be the reason of these clinical manifestations. A blood
specimen become then sent for your scientific laboratory for mutation screening in the
Huntingtin gene. Which of those strategies might satisfactory accomplish this assignment?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - ANS-Standard PCR
A affected person with impaired judgment, character modifications, signs and symptoms of
abnormal frame moves and melancholy comes to the medical doctor's office for a observe-up
go to. The doctor suspects a unmarried-gene disorder may be the reason of those clinical
manifestations. A blood specimen become then sent on your scientific laboratory for mutation
,screening inside the Huntington gene. Testing with fashionable PCR shows that affected person
has Huntington Disease, HD. Which of the subsequent would be regular with this diagnosis?
25 CAG repeats within the Huntington gene
eighty five CAG repeats within the Huntington gene
25 CGA repeats within the Huntington gene
85 CGA repeats inside the Huntington gene - ANS-eighty five CAG repeats in the Huntington
gene
A reverse dot blot is excellent performed with the aid of:
-Immobilizing a couple of unlabeled probes onto the membrane and then permitting one
categorized pattern to hybridize to the unlabeled probes
-Immobilizing one classified probe onto the membrane and permitting more than one
categorized samples to hybridize to the labeled probe
-Immobilizing multiple unlabeled probes onto the membrane and permitting more than one
unlabeled samples to hybridize to the unlabeled probes
-Allowing a unmarried classified pattern to hybridize to more than one categorized single
stranded probes onto the membrane - ANS--Immobilizing a couple of unlabeled probes onto the
membrane after which permitting one classified pattern to hybridize to the unlabeled probes
A technologist makes use of the spectrophotometer to quantify the quantity of DNA extracted
from a blood specimen diluted 1:30. The absorbance reading at 260 nm was discovered to be
2.545. If the absorbance at 280 nm gave a studying of one.406, and if the the DNA extract was
re-suspended in 0.800 mL of EDTA solution, the DNA yield is: - ANS-(2.545 * 50ng/mcl)=
127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( zero.800mL1000) =
3054 micrograms
A technologist makes use of the spectrophotometer to quantify the quantity of DNA extracted
from a blood specimen diluted 1:30. The absorbance studying at 260 nm changed into found to
be 2.545. The attention of DNA in this extract is:
76.35 micrograms/mL
3817.5 micrograms/mL
381.Seventy five micrograms/mL
3054.0 micrograms/mL - ANS-3817.5 micrograms/mL
According to Chargaff's rule of base pairing, adenine pairs with: - ANS-Thymine
According to the wobble hypothesis, a U within the 5' function of the anit-codon can pair with:
U or C
A or G
A, C or G
Only A - ANS-A or G
Acute promyelocytic leukemia (APL) is responsible for what translocation? - ANS-t(15;17)
After an amplification technique observed through gel electrophoresis, you took a image of your
gel. You be aware consistent bands in all of your gel lanes. However, you furthermore may see
, what appears to be a faint however great band in your reagent clean lane. What is your
satisfactory cause of the reagent blank band?
-Taq polymerase awareness turned into too low in this PCR reaction
-There became apparent infection in this PCR response
-The primers concentration changed into too high in this PCR reaction
-The dNTPs concentration changed into too excessive in this PCR reaction - ANS-There
became apparent contamination on this PCR reaction
All of the following are components of nucleic acids, EXCEPT:
Phosphate institution
Sugar (ribose or deoxyribose)
Nitrogenous base (A, G, C, T)
Formamide - ANS-Formamide
All of the following are liquid tumors, except:
Mantle cellular lymphoma
Ewing sarcoma
Burkitt's lymphoma
Acute promyelocytic leukemia - ANS-Ewing sarcoma
All of the subsequent are trinucleotide repeat issues, EXCEPT:
Spinocerebellar Ataxia Type eight
Huntington Disease
Asperger Disease
Fragile X Disease - ANS-Asperger Disease
All of the subsequent enzymes are part of the DNA replication equipment, EXCEPT:
Ligase
DNA polymerase
Luciferase
Helicase - ANS-Luciferase
All of the subsequent methods of amplification are considered goal amplification, besides:
Quantitative PCR
NASBA
Strand Displacement Amplification
Reverse Transcriptase PCR - ANS-NASBA
As a technologist running in a small clinical laboratory, a nasal swab specimen, suspected to be
colonized by using a spread of upper respiratory viruses, got here on your laboratory. You are
tasked with determining whether or not the specimen is colonized with rhinovirus, coronavirus,
and influenza. Which PCR technique would exceptional be appropriate for this challenge?
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