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BIOCHEM BRS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED R207,97
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BIOCHEM BRS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
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BIOCHEM BRS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED A bacterial mutant grows normally at 32°C but at 42°C accumulates short segments of newly synthesized DNA. Which one of the following enzymes is most likely to be defective at the nonpermissive temperature (the higher temp...

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  • November 14, 2024
  • 62
  • 2024/2025
  • Exam (elaborations)
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  • biochem brs exam questions and answers with comple

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BIOCHEM BRS EXAM QUESTIONS AND ANSWERS WITH

COMPLETE SOLUTIONS VERIFIED


A bacterial mutant grows normally at 32°C but at 42°C accumulates short

segments of newly synthesized DNA. Which one of the following enzymes is most

likely to be defective at the nonpermissive temperature (the higher temperature)

in this mutant?

(A) DNA primase

(B) DNA polymerase

(C) An exonuclease

(D) An unwinding enzyme (helicase)

(E) DNA ligase

(E) DNA ligase

An RNA produced from a fragment of DNA has the sequence of AAUUGGCU. The

sequence of the nontemplate strand in the DNA that gave rise to this sequence is

which one of the following?

(A) AGCCAATT

(B) AAUUGGCU

(C) AATTGGCT

(D) TTAACCGA

(E) UUAACCGA

(C) AATTGGCT

,Which one of the following changes in the coding region of an mRNA (caused by

a point mutation) would result in translation of a protein identical to the normal

protein?

(A) UCA → UAA

(B) UCA → CCA

(C) UCA → UCU

(D) UCA → ACA

(E) UCA → GCA

(C) UCA → UCU

Gene transcription rates and mRNA levels were determined for an enzyme that is

induced

by glucocorticoids. Compared with untreated levels, glucocorticoid treatment

caused a 10-fold increase in the gene transcription rate and a 20-fold increase in

both mRNA levels and enzyme activity. These data indicate that a primary effect

of glucocorticoid

treatment is to decrease which one of the following?

(A) The activity of RNA polymerase II

(B) The rate of mRNA translation

(C) The ability of nucleases to act on mRNA

(D) The rate of binding of ribosomes to mRNA

(E) The rate of transcription

(C) The ability of nucleases to act on mRNA

,A temperature-sensitive cell line would show early senescence when grown at the

nonpermissive temperature, and examination of the chromosomes demonstrated

many 3′ overhangs at the ends of the DNA fragments. The defective enzyme, at

the nonpermissive temperature, is which one of the following?

(A) Telomerase

(B) DNA ligase

(C) DNA polymerase

(D) A repair DNA polymerase

(E) A helicase

(A) Telomerase

A family, while on a picnic, picked some wild mushrooms to add to their picnic

salad. Shortly thereafter, all the members of the family became ill, with the

youngest child showing the most severe symptoms. The family is suffering these

effects owing to a primary inability to accomplish which one of the following in

their cells and tissues?

(A) Synthesize proteins

(B) Synthesize lipids

(C) Synthesize DNA

(D) Synthesize carbohydrates

(E) Repair damage in DNA

(A) Synthesize proteins

A newborn has found to be very photophobic, and his skin burns even with

minimal exposure to sunlight, eventually forming skin blisters. Neither parent

, exhibits this trait, although both are prone to burning when in the sun for a short

period of time. As the child grows, he is found to be at average height and weight

for his age, and is progressing normally along the developmental guidelines. He

is, however, kept inside at all times, and is carefully wrapped if he has to leave

the house. Fibroblasts isolated from this child are grown in culture, and in an

experiment, exposed to

UV light. An analysis of the fibroblast DNA will demonstrate which one of the

following?

(A) A preponderance of apurinic sites and apyrimidinic sites

(B) An increase in sister chromatid exchange rate

(C) A preponderance of abnormal base pairs in the DNA

(D) Loss of telomeres within the DNA

(E) An increase in cross-linked bases within the strands of DNA

(E) An increase in cross-linked bases within the strands of DNA

A 15-year-old boy was diagnosed with skin

cancer. He had always been sensitive to sunlight,

and had remained indoors for most of his life. An analysis of his DNA, from

isolated fibroblasts, indicated an increased level of thymine dimers when the

cells were exposed to UV light. The boy developed a skin tumor owing to an

increased mutation rate, which was caused by which one of the following?

(A) A lack of DNA primase activity

(B) Decreased recombination during mitosis

(C) Increased recombination during mitosis

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