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  5. Advanced Medical Microbiology (LSH4801)

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LSH4801 PORTFIOLIO (QUESTIONS & ANSWERS) 2024 - DUE 22 November 2024 ;100 % TRUSTED workings, Expert Solved, Explanations and Solutions. For assistance call or W.h.a.t.s.a.p.p us on ...(.+.2.5.4.7.7.9.5.4.0.1.3.2)........... QUESTION 1 [30] 1.1. Clinical laboratories routinely process specimens ...

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  • November 21, 2024
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  • 2024/2025
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  • lsh4801 portfiolio 2024 due 22 november 2024

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LSH4801
PORTFOLIO 2024
UNIQUE NO.
DUE DATE: 22 NOVEMBER 2024

, LSH4801

Portfolio 2024

Unique No.

Due Date: 22 November 2024

Advanced Medical Microbiology

Question 1

1.1 Immunofluorescence to Identify Streptococcus pyogenes

Application of Immunofluorescence:

Immunofluorescence is a diagnostic method that uses fluorescent-labeled antibodies to
detect specific antigens on microorganisms. To identify Streptococcus pyogenes:

 Antibody Preparation: Use fluorescent-labeled monoclonal antibodies
specifically designed to bind to antigens unique to S. pyogenes (e.g., the M
protein or group A carbohydrate antigen).
 Sample Preparation: Smear the isolated patient sample onto a glass slide and
fix the bacterial cells with heat or chemicals.
 Antibody Application: Add the fluorescent-labeled antibodies to the slide. After
incubation, unbound antibodies are washed off.
 Microscopic Examination: Use a fluorescence microscope to observe specific
binding. A positive identification of S. pyogenes will show fluorescence on the
bacterial cells.

Differentiation from Morphologically Similar Organisms:

1. Distinction from Other Streptococci:
o S. pyogenes belongs to Lancefield Group A. Perform a Lancefield
grouping test (serological grouping) to confirm the presence of group A
carbohydrate antigen.

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