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Summary Microbiology 214: Control of microorganisms & Antimicrobial chemotherapy R120,00
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Summary Microbiology 214: Control of microorganisms & Antimicrobial chemotherapy

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These notes cover Chapter 8 & 9 from the textbook. The topics covered are 1) Control of microorganisms in the environment and 2) Antimicrobial chemotherapy. Disclaimer: These notes have been made from the listed resources and are not all my own intellectual property. I have simply summarised clas...

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  • June 3, 2024
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camrynjordaan
terminology

sterilization destruction
ofall viablecellssporesalellularentities
Disinfection destruction inhibitionremovalof microorganisms notsportfrom inanimatesurfalls
vanitization reovitionof microbial popto level whichreachpublicstandards
Antiseplis Removal Destructioninhibition
of microbes from livingtissueto prevent wound infection

Microbialcontrol Mechanical Physical Chemical Biological


terilants AutoclaveTyndallilationUATmembrane Airfiltration EtogasCeagasAldehydesToxinsNitin
mechanical


Filtration

traps particles
porous filters 0,20mtrapsspores cells
HEPA Nasfilters usedforair filtration

phyrical

moistheat Dryheat

denatures proteinsdegradenucleicacidsdisruptscell membranes lesseffective i Needs highertemps for
longerexposure time
Pasteurization storetop dehydrates cell
50 600 30min oxidised cellularconstituents

delaysfoodspoilagebyslowinggrowth denaturesproteins



FlashPasteurization HIST Radiation
7200 15min
UV Ionizing
xraysGamma
hightempsterilization
Ultra UHI aire
only surfaces deeppenetration
140 1500 135 stemmed
shallow
penetration
HH in containerforuptogodays dimers
thyminethymine lethal
inhibitsDNA
rep
Tyndallisation mutations
intermittant sterilization

steam60min
intubate 24hr
Repeat3x

g cannedfood

Autoclave


29Mnookpa
to12min

, Chemical


phenolics
Phenolderivatives lesstoxic p ersistantmore
irritating more stable
ring
Aromatic s Wsubstituents
cellmembranes
Denatureproteinsdisrupt


Alcohols

ethanol isopropanol

701Alcohol 301watermosteffective
lipidsdenatures proteinsdegradessurfacetension
degradesmembrane



Halogens



t.fmestpfeih'sioiiioiisdetcens
Iodophors Betaine nonstaining lessirritating
Bleach
eartffff.LY
p
Heavy metals oligodynamic


inactivate haltsmetabolism enzymaticactivity
precipitate proteins
byreactingw sulfhydrylgros
Mercurochrome
Agnos


QuaternaryAmmoniumcompounds



intra cationicnuorophilichead
ftp.tnsYfiYpmimfhhipatnio
Aldehydes


i
374 o to
iftoxins
acids
inactivate proteins nucleic
irritating




Gases

to penetrates packagessterilizesheat sensitivematerial
Reacts w DNA proteins NOrep enzymeactivity

Biological


Bacteriophage vibrio
Boello Nisin toxin

virusinfectsbacteria Bacterial predator no wig dehydration
noPMF noAed
Attachment Attacksprey
pygmy
penetration formsboelloplast in wall
Biosynthesis matures kills cell
maturation
lysis

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