MCB2021F (MCB2021F)

Learning objectives for biochemistry MCB2021F

Gene cloning

This module will guide you through the process of generating a gene library and screening the library for a specific gene of interest.

By the end of this module, you should Understand nucleotide structures How nucleotides are joined together to form nucleic acids How information is stored in and transferred via nucleic acids Understand the biological functions of nucleotides and nucleic acids

This module discusses the general properties of enzymes and their function as biological catalysts, the study of chemical reactions catalysed by enzymes using enzyme kinetics, the analysis of enzyme inhibitors using enzyme kinetics, the basic principles of catalysis and the regulation of enzymes.

This module discusses the primary structure (the amino acid sequence) of proteins, how the primary structure of a protein can be determined by Edman degradation, and how proteins can be identified in samples using mass spectrometry

This module discusses secondary, tertiary and quaternary structure of proteins.

understand the principle underlying size exclusion chromatography. understand the terms elution volume, void volume, intrinsic volume and penetrable volume in size exclusion chromatography.

This module covers general properties of amino acids, stereochemistry of amino acids, acid/base properties of amino acids and analysis of amino acids.

Contents on the first page.